Academic Journal
A validated LC-MS/MS method for the simultaneous quantification of iothalamate and hippuran in serum and urine for non-radioactive kidney function assessment
| العنوان: | A validated LC-MS/MS method for the simultaneous quantification of iothalamate and hippuran in serum and urine for non-radioactive kidney function assessment |
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| المؤلفون: | Mohamed, Abdulfataah A.A., Walland, Peter, Stevens, Jasper, van Londen, Marco, Heerspink, Hiddo J.L., Gansevoort, Ron T., van de Merbel, Nico C. |
| المصدر: | Mohamed , A A A , Walland , P , Stevens , J , van Londen , M , Heerspink , H J L , Gansevoort , R T & van de Merbel , N C 2024 , ' A validated LC-MS/MS method for the simultaneous quantification of iothalamate and hippuran in serum and urine for non-radioactive kidney function assessment ' , Journal of Chromatography B , vol. 1247 , 124329 . https://doi.org/10.1016/j.jchromb.2024.124329 |
| سنة النشر: | 2024 |
| المجموعة: | University of Groningen research database |
| مصطلحات موضوعية: | IOTHALAMATE, Hippuran, exogenous filtration marker, non-radioactive, kidney function, mGFR, ERPF, LC-MS/MS, validation of a method |
| الوصف: | A novel liquid chromatography-tandem mass spectrometry method is described for the quantitative determination of the kidney function markers iothalamate and hippuran in human serum and urine. It is based on protein precipitation with methanol followed by dilution of the supernatant for serum and simple dilution for urine. The polar analytes are chromatographically separated by a 6.5-min gradient on a low-ligand density reversed-phase column; detection is performed by electrospray ionization tandem mass spectrometry in the positive ion mode against stable-isotope labeled internal standards. The results of a thorough method validation show that iothalamate and hippuran can be simultaneously quantified in the concentration ranges 0.500–30.0 ng/mL and 10.0–5000 ng/mL for serum and urine, respectively, with values for CV and absolute bias not exceeding 10 %, and with sufficient stability in all relevant matrices and solvents. The method was successfully applied for the analysis of serum and urine samples of multiple individuals who received both iothalamate and hippuran. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1016/j.jchromb.2024.124329 |
| الاتاحة: | https://hdl.handle.net/11370/bbc05c3d-379f-4abe-aaab-5b343ba4bad7 https://research.rug.nl/en/publications/bbc05c3d-379f-4abe-aaab-5b343ba4bad7 https://doi.org/10.1016/j.jchromb.2024.124329 https://pure.rug.nl/ws/files/1153084001/Mohamed_et_al_LCMSMS_iothalamate_and_hippuran_serum_urine_JChromB_2024.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.BDB159D9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jchromb.2024.124329 |
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