التفاصيل البيبلوغرافية
| العنوان: |
Improving the Thermo-Activity and -Stability of Pectate Lyase from Dickeya dadantii DCE-01 for Ramie Degumming |
| المؤلفون: |
Huan Xu, Shengwen Duan, Xiangyuan Feng, Qi Yang, Ke Zheng, Yuande Peng, Lifeng Cheng |
| المصدر: |
Processes; Volume 9; Issue 12; Pages: 2106 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2021 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
Dickeya dadantii DCE-01, pectate lyase, site-directed mutagenesis, thermal stability, degumming |
| جغرافية الموضوع: |
agris |
| الوصف: |
To improve the thermal stability of pectate lyase for ramie degumming, we modified the novel pectate lyase gene (pelG403) derived from the Dickeya dadantii DCE-01 high-efficiency ramie degumming strain by site-directed mutagenesis. Twelve mutants were acquired, wherein a prospective mutant (A129V) showed better enzyme activity and thermal stability. Compared with the wild type (PelG403), the specific enzyme activity and the optimal reaction temperature of A129V in the fermentation broth increased by 20.1%, and 5 °C, respectively. Under the conditions of 55 °C and pH 9.0, the weightlessness rate of ramie raw materials of A129V increased by 6.26%. Therefore, this study successfully improved the enzyme activity and heat resistance of PelG403 in an alkaline environment, which may contribute to the development of enzyme preparations and the elucidation of the mechanism for ramie bio-degumming. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Biological Processes and Systems; https://dx.doi.org/10.3390/pr9122106 |
| DOI: |
10.3390/pr9122106 |
| الاتاحة: |
https://doi.org/10.3390/pr9122106 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.BDA0D97C |
| قاعدة البيانات: |
BASE |