Academic Journal
AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the regulation of sperm motility
| العنوان: | AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the regulation of sperm motility |
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| المؤلفون: | Violeta Calle-Guisado, Ana Hurtado de Llera, David Martin-Hidalgo, Jose Mijares, Maria C Gil, Ignacio S Alvarez, Maria J Bragado, Luis J Garcia-Marin |
| المصدر: | Asian Journal of Andrology, Vol 19, Iss 6, Pp 707-714 (2017) |
| بيانات النشر: | Wolters Kluwer Medknow Publications |
| سنة النشر: | 2017 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | AMP-activated kinase, human spermatozoa, immunolocalization, sperm motility, sperm quality, Diseases of the genitourinary system. Urology, RC870-923 |
| الوصف: | AMP-activated kinase (AMPK), a protein that regulates energy balance and metabolism, has recently been identified in boar spermatozoa where regulates key functional sperm processes essential for fertilization. This work′s aims are AMPK identification, intracellular localization, and their role in human spermatozoa function. Semen was obtained from healthy human donors. Sperm AMPK and phospho-Thr172-AMPK were analyzed by Western blotting and indirect immunofluorescence. High- and low-quality sperm populations were separated by a 40%-80% density gradient. Human spermatozoa motility was evaluated by an Integrated Semen Analysis System (ISAS) in the presence or absence of the AMPK inhibitor compound C (CC). AMPK is localized along the human spermatozoa, at the entire acrosome, midpiece and tail with variable intensity, whereas its active form, phospho-Thr172-AMPK, shows a prominent staining at the acrosome and sperm tail with a weaker staining in the midpiece and the postacrosomal region. Interestingly, spermatozoa bearing an excess residual cytoplasm show strong AMPK staining in this subcellular compartment. Both AMPK and phospho-Thr172-AMPK human spermatozoa contents exhibit important individual variations. Moreover, active AMPK is predominant in the high motility sperm population, where shows a stronger intensity compared with the low motility sperm population. Inhibition of AMPK activity in human spermatozoa by CC treatment leads to a significant reduction in any sperm motility parameter analyzed: percent of motile sperm, sperm velocities, progressivity, and other motility coefficients. This work identifies and points out AMPK as a new molecular mechanism involved in human spermatozoa motility. Further AMPK implications in the clinical efficiency of assisted reproduction and in other reproductive areas need to be studied. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1008-682X 1745-7262 |
| Relation: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2017;volume=19;issue=6;spage=707;epage=714;aulast=; https://doaj.org/toc/1008-682X; https://doaj.org/toc/1745-7262; https://doaj.org/article/49a3f0e2838d4edbb849da73220e6243 |
| DOI: | 10.4103/1008-682X.185848 |
| الاتاحة: | https://doi.org/10.4103/1008-682X.185848 https://doaj.org/article/49a3f0e2838d4edbb849da73220e6243 |
| رقم الانضمام: | edsbas.BD9C01DC |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1008682X 17457262 |
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| DOI: | 10.4103/1008-682X.185848 |