Academic Journal
The Landscape of L1 Retrotransposons in the Human Genome Is Shaped by Pre-insertion Sequence Biases and Post-insertion Selection
| العنوان: | The Landscape of L1 Retrotransposons in the Human Genome Is Shaped by Pre-insertion Sequence Biases and Post-insertion Selection |
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| المؤلفون: | Sultana, Tania, van Essen, Dominic, Siol, Oliver, Bailly-Bechet, Marc, Philippe, Claude, El Aabidine, Amal Zine, Pioger, Léo, Nigumann, Pilvi, Saccani, Simona, Andrau, Jean-Christophe, Gilbert, Nicolas, Cristofari, Gael |
| المساهمون: | Institut de Recherche sur le Cancer et le Vieillissement (IRCAN), Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA), Université Côte d'Azur (UniCA), Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique Moléculaire de Montpellier (IGMM), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut Pasteur de Shanghai, Académie des Sciences de Chine - Chinese Academy of Sciences (IPS-CAS), Pasteur Network (Réseau International des Instituts Pasteur), Fondation pour la Recherche Medicale FRM-DEP20131128533, European Research Council ERC-2009-StG 243312, Agence Nationale de la Recherche (LABEX SIGNALIFE) ANR-11-LABX-0028-01, Agence Nationale de la Recherche (RETROMET) ANR-16-CE12-0020, Canceropole PACA (Projet Emergence), CNRS GDR 3546, University Hospital Federation (FHU) OncoAge, FEDER, Inserm, Universite Cote d'Azur (France), University of Dhaka (Bangladesh), Agence Nationale de la Recherche (RETROGENO) ANR-12-BSV6-0003, Region Provence Alpes Cote d'Azur, Conseil Departemental 06, ITMO Cancer Aviesan plan cancer, ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011), ANR-16-CE12-0020,RETROMET,Rendre unique l'ADN répété ou comment révéler la régulation épigénétique des rétrotransposons L1 dans les cellules somatiques humaines à une résolution inégalée.(2016), ANR-12-BSV6-0003,RETROGENO,Complexes de rétrotransposition du LINE-1 humain et instabilité génomique(2012) |
| المصدر: | ISSN: 1097-2765. |
| بيانات النشر: | CCSD Cell Press |
| سنة النشر: | 2019 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | LINE1, LINE-1 retrotransposition, reverse transcription, human-cells, replication, dna, intergration site selection, transposable elements, [SDV]Life Sciences [q-bio], [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] |
| الوصف: | International audience ; L1 retrotransposons are transposable elements and major contributors of genetic variation in humans. Where L1 integrates into the genome can directly impact human evolution and disease. Here, we experimentally induced L1 retrotransposition in cells and mapped integration sites at nucleotide resolution. At local scales, L1 integration is mostly restricted by genome sequence biases and the specificity of the L1 machinery. At regional scales, L1 shows a broad capacity for integration into all chromatin states, in contrast to other known mobile genetic elements. However, integration is influenced by the replication timing of target regions, suggesting a link to host DNA replication. The distribution of new L1 integrations differs from those of preexisting L1 copies, which are significantly reshaped by natural selection. Our findings reveal that the L1 machinery has evolved to efficiently target all genomic regions and underline a predominant role for post-integrative processes on the distribution of endogenous L1 elements. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/30956044; PRODINRA: 479672; PUBMED: 30956044; WOS: 000466703900015 |
| DOI: | 10.1016/j.molcel.2019.02.036 |
| الاتاحة: | https://hal.science/hal-02401618 https://hal.science/hal-02401618v1/document https://hal.science/hal-02401618v1/file/S1097276519301479.pdf https://doi.org/10.1016/j.molcel.2019.02.036 |
| Rights: | http://creativecommons.org/licenses/by-nc/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.BD256A13 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.molcel.2019.02.036 |
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