Academic Journal
Dynamic BTB-domain filaments promote clustering of ZBTB proteins
| العنوان: | Dynamic BTB-domain filaments promote clustering of ZBTB proteins |
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| المؤلفون: | Mance, Lucija, Bigot, Nicolas, Zhamungui Sánchez, Edison, Coste, Franck, Martín-González, Natalia, Zentout, Siham, Biliškov, Marin, Pukało, Zofia, Mishra, Aanchal, Chapuis, Catherine, Arteni, Ana-Andreea, Lateur, Axelle, Goffinont, Stéphane, Gaudon, Virginie, Talhaoui, Ibtissam, Casuso, Ignacio, Beaufour, Martine, Garnier, Norbert, Artzner, Franck, Cadene, Martine, Huet, Sébastien, Castaing, Bertrand, Suskiewicz, Marcin, Józef |
| المساهمون: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes (Biosit : Biologie - Santé - Innovation Technologique), Turing Centre for Living Systems Marseille (TCLS), Architecture et fonction des macromolécules biologiques (AFMB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut de Physique de Rennes (IPR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Institut universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Ligue contre le Cancer CSIRGO 2022, CNRS Chimie, ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), European Project: SyMBioMS, European Project: 101078837,SUMOwriteNread, European Project: 2017-EX002979 |
| المصدر: | ISSN: 1097-2765. |
| بيانات النشر: | CCSD Cell Press |
| سنة النشر: | 2024 |
| المجموعة: | Université François-Rabelais de Tours: HAL |
| مصطلحات موضوعية: | Protein filaments, Protein polymerisation, Nuclear foci, Transcription factors, Gene repression, ZBTB proteins, ZBTB8A, ZBTB18, ZBTB26, NACC1, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; The formation of dynamic protein filaments contributes to various biological functions by clustering individual molecules together and enhancing their binding to ligands. We report such a propensity for the BTB domains of certain proteins from the ZBTB family, a large eukaryotic transcription factor family implicated in differentiation and cancer. Working with Xenopus laevis and human proteins, we solved crystal structures of filaments formed by dimers of BTB domains of ZBTB8A and ZBTB18 and demonstrated concentration-dependent higher-order assemblies of these dimers in solution. In cells, the BTB-domain filamentation supports clustering of full-length human ZBTB8A and ZBTB18 into dynamic nuclear foci and contributes to the ZBTB18-mediated repression of a reporter gene. The BTB domains of up to 21 human ZBTB-family members and two related proteins, NACC1 and NACC2, are predicted to behave in the same manner. Our results suggest that filamentation is a more common feature of transcription factors than currently appreciated. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38996459; info:eu-repo/grantAgreement//101078837/EU/Mechanisms of protein SUMOylation and its functional consequences/SUMOwriteNread; info:eu-repo/grantAgreement//2017-EX002979/EU/European FEDER funds/; PUBMED: 38996459 |
| DOI: | 10.1016/j.molcel.2024.05.029 |
| الاتاحة: | https://hal.science/hal-04631262 https://hal.science/hal-04631262v1/document https://hal.science/hal-04631262v1/file/Mance_et_al_combined_accepted.pdf https://doi.org/10.1016/j.molcel.2024.05.029 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.BD013389 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.molcel.2024.05.029 |
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