التفاصيل البيبلوغرافية
| العنوان: |
Immunization with antigenic extracts of Leishmania associated with Montanide ISA 763 adjuvant induces partial protection in BALB/c mice against Leishmania (Leishmania) amazonensis infection |
| المؤلفون: |
Cargnelutti, Diego Esteban, Salomón, María Cristina, Celedon, Verónica, Garcia Bustos, Maria Fernanda, Morea, Gastón, Cuello Carrión, Fernando Darío, Scodeller, Eduardo |
| بيانات النشر: |
Elsevier |
| المجموعة: |
CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: |
LEISHMANIA AMAZONENSIS, MONTANIDE ISA 763, VACUNAS, ADYUVANTES, BALB/c, R848, https://purl.org/becyt/ford/1.6, https://purl.org/becyt/ford/1 |
| الوصف: |
Background/Purpose: A proper adjuvant has a relevant role in vaccine formulations to generate an effective immune response. In this study, total Leishmania antigen (TLA) formulated with Montanide ISA 763 or R848 as adjuvants were evaluated as a first generation Leishmania vaccine in a murine model. Methods: Immunization protocols were tested in BALB/c mice with a subcutaneous prime/boost regimen with an interval of 3 weeks. Mice immunized with unadjuvanted TLA and phosphate-buffered saline (PBS) served as control groups. On Day 21 and Day 36 of the protocol, we evaluated the humoral immune response induced by each formulation. Fifteen days after the boost, the immunized mice were challenged with 1 × 105 promastigotes of Leishmania (Leishmania) amazonensis in the right footpad (RFP). The progress of the infection was followed for 10 weeks; at the end of this period, histopathological studies were performed in the RFP. Results: Vaccines formulated with Montanide ISA 763 generated an increase in the production of immunoglobulin G (IgG; p < 0.05) compared with the control group. There were no statistically significant differences in IgG1 production between the study groups. However, immunization with TLA-Montanide ISA 763 resulted in an increase in IgG2a compared to the unadjuvanted control (p < 0.001). Also noteworthy was the fact that a significant reduction in swelling and histopathological damage of the RFP was recorded with the Montanide ISA 763 formulation. Conclusion: We conclude that the immunization of BALB/c mice with a vaccine formulated with TLA and Montanide ISA 763 generated a protective immune response against L. (L.) amazonensis, characterized by an intense production of IgG2a. ; Fil: Cargnelutti, Diego Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo; Argentina ; Fil: Salomón, María Cristina. Universidad Nacional de Cuyo; Argentina ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| تدمد: |
1684-1182 |
| Relation: |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1684118214000097; http://hdl.handle.net/11336/32203; Cuello Carrión, Fernando Darío; Cargnelutti, Diego Esteban; Garcia Bustos, Maria Fernanda; Salomón, María Cristina; Celedon, Verónica; Morea, Gastón; et al.; Immunization with antigenic extracts of Leishmania associated with Montanide ISA 763 adjuvant induces partial protection in BALB/c mice against Leishmania (Leishmania) amazonensis infection; Elsevier; Journal of Microbiology Immunology and Infection; 49; 1; 3-2014; 7-9; CONICET Digital; CONICET |
| الاتاحة: |
http://hdl.handle.net/11336/32203 |
| Rights: |
info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
| رقم الانضمام: |
edsbas.BCC8C5A3 |
| قاعدة البيانات: |
BASE |