التفاصيل البيبلوغرافية
| العنوان: |
MITOL interacts with PolγA. |
| المؤلفون: |
Mansoor Hussain (10225136), Aftab Mohammed (10225139), Shabnam Saifi (10225142), Aamir Khan (3467267), Ekjot Kaur (10225145), Swati Priya (10225148), Himanshi Agarwal (10225151), Sagar Sengupta (1910200) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Genetics, Evolutionary Biology, Developmental Biology, Infectious Diseases, Virology, Environmental Sciences not elsewhere classified, PEO mutants, PEO patients, RNF, K 6 linkage, WT, mitochondrial entry, Lysine 1060 residue, MITOL, DNA, mtDNA, mitochondrial E 3 ligase, Pol |
| الوصف: |
(A, B) MITOL overexpression leads to decreased levels of PolγA. Whole cell extracts were made from HEK293T overexpressing the indicated mitochondrial E3 ligases. Western blot analysis was carried out with the indicated antibodies. The relative levels of PolγA to hsp60 in (A) have been quantitated from 3 biological replicates. (C) MITOL causes proteasomal degradation of PolγA. Whole cell extracts were made from HEK293T cells overexpressing Myc MITOL for 24 hours and grown either in absence or presence of MG132. Western blot analysis was carried out with the indicated antibodies. The relative levels of PolγA to hsp60 have been quantitated from 3 biological replicates. (D) Catalytic activity of MITOL is essential for the proteasomal degradation of PolγA. Whole cell extracts were made from HEK293T cells overexpressing either Myc MITOL WT or CD. Western blot analysis was carried out with the indicated antibodies. The relative levels of PolγA to hsp60 have been quantitated from 3 biological replicates. (E–G) Depletion of MITOL stabilizes PolγA. Whole cell extracts were made from (E) HEK 293T cells transfected with either siControl or siMITOL #1 or siMITOL #2 (F) HeLa shGFP and Hela shMITOL cells or from (G) MITOL flox/flox cells either untreated or treated with 4-OHT for 4 days. Western blot analysis was carried out with the indicated antibodies. For all 3 experiments, the relative levels of PolγA to hsp60 have been quantitated from 3 biological replicates. (H, I) Half-life of PolγA increases in absence of MITOL. (H) HEK293T cells transfected with either siControl or siMITOL #1. After 24 hours of transfection, cells were grown either without CHX treatment or after CHX treatment for the indicated hours (hrs), and whole cell extracts were made. Western blot analysis was carried out with the indicated antibodies. (I) The percentage of PolγA remaining after CHX treatment has been quantitated. The quantification is from 3 biological replicates. (J) PolγA interact with MITOL in vivo . (Top) Whole cell extracts were made ... |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/MITOL_interacts_with_Pol_A_/14156036 |
| DOI: |
10.1371/journal.pbio.3001139.g001 |
| الاتاحة: |
https://doi.org/10.1371/journal.pbio.3001139.g001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.BCC653AA |
| قاعدة البيانات: |
BASE |