Academic Journal
Revealing the specificity of human H1 influenza A viruses to complex N-glycans
| العنوان: | Revealing the specificity of human H1 influenza A viruses to complex N-glycans |
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| المؤلفون: | Canales, A., Sastre, Javier, Orduña, José M., Spruit, Cindy M., Pérez-Castells, Javier, Domínguez, Gema, Bouwman, Kim M., Van der Woude, Roosmarijn, Cañada, F. Javier, Nycholat, Corwin M., Paulson, James C., Boons, Geert-Jan, Jiménez-Barbero, Jesús, De Vries, Robert P. |
| المساهمون: | European Commission, Royal Dutch Academy of Sciences, he Netherlands Organization for Scientific Research, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Instituto de Salud Carlos III, Canales, A., Sastre, Javier, Orduña, José M., Spruit, Cindy M., Pérez-Castells, Javier, Domínguez, Gema, Bouwman, Kim M., Van der Woude, Roosmarijn, Cañada, F. Javier, Nycholat, Corwin M., Paulson, James C., Boons, Geert-Jan, Jiménez-Barbero, Jesús, De Vries, Robert P. |
| بيانات النشر: | American Chemical Society |
| سنة النشر: | 2023 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Influenza virus, N-glycan, Recognition, Glycan array, NMR |
| الوصف: | 11 p.-5 fig.-1 schem.-1 graph. abst. ; Influenza virus infection remains a threat to human health since viral hemagglutinins are constantly drifting, escaping infection and vaccine-induced antibody responses. Viral hemagglutinins from different viruses display variability in glycan recognition. In this context, recent H3N2 viruses have specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc). In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak. We also analyzed one engineered H6N1 mutant to understand if the preference for tri-LacNAc motifs could be a general trend in human-type receptor-adapted viruses. In addition, we developed a new NMR approach to perform competition experiments between glycans with similar compositions and different lengths. Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs. ; R.P.d.V. is a recipient of an ERC Starting grant from the European Commission (802780) and a Beijerinck Premium of the Royal Dutch Academy of Sciences. The glycan array setup was supported by the Netherlands Organization for Scientific Research (NWO, TOP-PUNT 718.015.003 to G.-J.P.H.B.). Dr. Lin Liu (CCRC) and Dr. Margreet A. Wolfert (Utrecht University) developed, printed, and validated the glycan microarray. We would like to thank Nikoloz Nemanichvili for technical assistance. A.C. acknowledges funding from Agencia Estatal de Investigación “Spanish Ministry of Science and Innovation” (MICINN) project PID2019-105237GB-I00. J.P.C. acknowledges funding by the Spanish MICINN, grant no. RTI2018-095588-B-I00 (co-funded by the European Regional Development Fund/European Social Fund, “Investing in your future”). JJB also thanks funding by the European Research Council (RECGLYCANMR, ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0002-7863 1520-5126 |
| Relation: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/EC/H2020/802780; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105237GB-I00/ES/NUEVAS METODOLOGIAS DE RMN PARA ESTUDIAR N-GLICANOS Y GLICOPROTEINAS/; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095588-B-I00/ES/REACCIONES DE CICLOADICION Y CARBONILACION EN REACTOR DE FLUJO. NUEVA REACTIVIDAD DE CICLOPROPENOS. SINTESIS DE CARBOHIDRATOS CON CENTROS PARAMAGNETICOS PARA ESTUDIOS POR RMN/; info:eu-repo/grantAgreement/EC/H2020/788143; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094751-B-C21/ES/RMN Y RECONOCIMIENTO MOLECULAR DE CARBOHIDRATOS: LECTINAS, ENZIMAS Y ACIDOS NUCLEICOS/; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094751-B-C22/ES/RMN Y RECONOCIMIENTO MOLECULAR DE CARBOHIDRATOS: LECTINAS, ENZIMAS Y ACIDOS NUCLEICOS/; Publisher's version; https://doi.org/10.1021/jacsau.2c00664; Sí; Journal of the American Chemical Society (2023); http://hdl.handle.net/10261/296859 |
| DOI: | 10.1021/jacsau.2c00664 |
| الاتاحة: | http://hdl.handle.net/10261/296859 https://doi.org/10.1021/jacsau.2c00664 |
| Rights: | open |
| رقم الانضمام: | edsbas.BC733D21 |
| قاعدة البيانات: | BASE |
| تدمد: | 00027863 15205126 |
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| DOI: | 10.1021/jacsau.2c00664 |