Academic Journal
Measurement of soluble Fcγ receptor type IIIa derived from macrophages in plasma: increase in patients with rheumatoid arthritis
| العنوان: | Measurement of soluble Fcγ receptor type IIIa derived from macrophages in plasma: increase in patients with rheumatoid arthritis |
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| المؤلفون: | MASUDA, M, MORIMOTO, T, KOBATAKE, S, NISHIMURA, N, NAKAMOTO, K, DONG, X H, KOMIYAMA, Y, OGAWA, R, TAKAHASHI, H |
| المصدر: | Clinical and Experimental Immunology ; volume 132, issue 3, page 477-484 ; ISSN 0009-9104 1365-2249 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2003 |
| الوصف: | SUMMARY FcγRIII (CD16) is found in two alternative forms, a transmembrane FcγRIIIa expressed on NK cells and macrophages, and a glycosylphosphatidylinositol-linked FcγRIIIb present on neutrophils. Previously, we measured soluble FcγRIIIa (sFcγRIIIa) in plasma of NA(1 +, 2-) phenotyped donors with the anti-FcγRIII monoclonal antibody (MoAb) GRM1, which recognizes NA2-FcγRIIIb and FcγRIIIa. The level of sFcγRIIIa, as well as the total sFcγRIII (sFcγRIIIa plus sFcγRIIIb) in patients with rheumatoid arthritis (RA) was significantly higher than that in healthy controls. In this study, we measured sFcγRIIIaMφ in plasma with a newly developed anti-FcγRIII MoAb, MKGR14 (mIgM), which recognizes FcγRIIIaMφ specifically. From the recovery of purified sFcγRIIIaMφ, the amount of sFcγRIIIaMφ present was about half that of sFcγRIIIaNK, and that of sFcγRIIIa was about 50 times lower than that of sFcγRIIIb in pooled plasma from healthy NA(1 +, 2-) phenotyped donors. The level of sFcγRIIIaMφ in RA patients was about four times higher than that in healthy controls. In RA patients, both the sFcγRIIIaMφ and sFcγRIIIa levels were increased as proportionally as the Lansbury Index. The sFcγRIIIa, but not sFcγRIIIaMφ levels, were increased directly proportional to C-reactive protein. sFcγRIIIaMφ may be a novel marker of disease activity in RA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1046/j.1365-2249.2003.02168.x |
| الاتاحة: | https://doi.org/10.1046/j.1365-2249.2003.02168.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2249.2003.02168.x https://academic.oup.com/cei/article-pdf/132/3/477/42142141/j.1365-2249.2003.02168.x.pdf |
| Rights: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| رقم الانضمام: | edsbas.BC5FDB2B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1046/j.1365-2249.2003.02168.x |
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