التفاصيل البيبلوغرافية
| العنوان: |
Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation |
| المؤلفون: |
David M. Engelthaler, Tom Chiller, Joshua Colvin, James S. Beckstrom-sternberg, Alexis Christoforides, John V. Pearson, John Carpten, Paul Keim, Ashley Peterson, Dawn Terashita, S. Arunmozhi Balajee, S. M. Beckstrom-sternberg, E. M. Driebe, T. Moses, W. Tembe, A. Christoforides, J. V. Pearson, J. Carpten |
| المساهمون: |
The Pennsylvania State University CiteSeerX Archives |
| المصدر: |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/80/6d/Emerg_Infect_Dis_2011_Feb_17(2)_227-232.tar.gz |
| المجموعة: |
CiteSeerX |
| الوصف: |
Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confi rm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 singlenucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefi ts to public health for investigation of clusters considered to be linked to a single source. Genotyping of microorganisms typically relies on comparison of genomic features (e.g., fragment size, repeats, single-nucleotide polymorphisms [SNPs]) between strains and/or against a database of feature profiles (e.g., PulseNET and mlst.net) for a population of the microbe of Author affi liations: Translational Genomics Research Institute |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/zip |
| اللغة: |
English |
| Relation: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.288.2382 |
| الاتاحة: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.288.2382 |
| Rights: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: |
edsbas.BC45BE67 |
| قاعدة البيانات: |
BASE |