Book
Development of an integrated platform for multi-omics prototyping of Pseudomonas putida strains
| العنوان: | Development of an integrated platform for multi-omics prototyping of Pseudomonas putida strains |
|---|---|
| المؤلفون: | Gurdo, Nicolas |
| المصدر: | Gurdo , N 2023 , Development of an integrated platform for multi-omics prototyping of Pseudomonas putida strains . Technical University of Denmark . |
| بيانات النشر: | Technical University of Denmark |
| سنة النشر: | 2023 |
| المجموعة: | Technical University of Denmark: DTU Orbit / Danmarks Tekniske Universitet |
| الوصف: | Cutting-edge, systems-level approaches to study the physiology and metabolism of microorganisms can bridge the gap between fundamental scientific knowledge and practical biotechnological applications. Such technologies utilize advanced methods and interdisciplinary strategies that integrate fields as diverse as synthetic and systems biology, multi-omics, big data, and computational biology. Through the application of these tools, researchers can gain a comprehensive understanding of a biological systems and their underlying mechanisms. This knowledge can then be translated into tangible solutions, including therapeutics, enhanced bioprocessing techniques, and sustainable biotechnological applications. In the intersection of fundamental and applied knowledge, systems biology and multi-omics analysis play a pivotal role in unraveling the complexity of biological systems. Pseudomonas putida is a robust microbial chassis endowed with metabolic versatility, adaptability, and genetic tractability, making it an ideal model organism for studying complex biological networks and developing biotechnological applications. Integrating systems biology and multi-omics approaches in P. putida research will provide valuable insights into cellular functions and will support the design of tailored metabolic engineering strategies for several biotechnological purposes. The work reported in this thesis builds on the versatile metabolism of P. putida and describes a multi-omics workflow developed to explore the physiology and metabolism of this model bacterium. Hence, a multi-omics platform was established to enable the analysis of the metabolome, proteome, and fluxome of P. putida. Furthermore, to extend the utility of the platform, a quantitative proteomic approach incorporating QconCATs was integrated into the workflow. This platform in combination with physiological analysis, adaptive laboratory evolution approaches and genomics was able to explore P. putida metabolism in multi-substrate environments (glycolytic and ... |
| نوع الوثيقة: | book |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://orbit.dtu.dk/en/publications/567fa4a8-a8aa-409b-8259-1de36b135ace |
| الاتاحة: | https://orbit.dtu.dk/en/publications/567fa4a8-a8aa-409b-8259-1de36b135ace https://backend.orbit.dtu.dk/ws/files/350695785/Final_version_PhD_Thesis_Nicols_Gurdo_02082023.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.BC11AF2C |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |