التفاصيل البيبلوغرافية
| العنوان: |
C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways |
| المؤلفون: |
Chen, Yan, Liu, Yang, Li, Hao, Huna, Risu, Tan, Xiaohan, Li, Ning, Zhang, Yiying, Jiao, Xiaohui, Liu, Mingyue |
| المصدر: |
Journal of Applied Oral Science; Vol. 31 (2023); e20220404 ; Journal of Applied Oral Science; v. 31 (2023); e20220404 ; 1678-7765 ; 1678-7757 |
| بيانات النشر: |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
| سنة النشر: |
2023 |
| المجموعة: |
Universidade de São Paulo: Portal de Revistas da USP |
| مصطلحات موضوعية: |
C5aR antagonist, Gingival fibroblasts, Periodontitis, NF-κB, MAPK |
| الوصف: |
Objective: Abnormal complement activation is associated with periodontitis. W54011 is a novel non-peptide C5aR antagonist (C5aRA) that exhibits favorable anti-inflammatory effects in various inflammatory models. However, whether W54011 inhibits periodontitis has not yet been fully elucidated. To address this, we have investigated the probable anti-inflammatory mechanism of W54011 in LPS-treated inflammation in human gingival fibroblasts (HGFs). Methodology: HGFs were isolated from healthy gingival tissue samples using the tissue block method and were identified with immunofluorescence staining. The CCK8 assay and reverse transcription-PCR (RT-PCR) were used to select the optimal induction conditions for Lipopolysaccharide (LPS) and C5aRA (according to supplementary data S1, S2 and S3). The levels of inflammatory cytokines, C5aR, and the activation of NF-κB/MAPK signaling pathways were determined by RT-quantitative PCR (RT-qPCR) and Western blotting. Results: Immunofluorescence results showed that vimentin and FSP-1 were positive in HGFs and Keratin was negative in HGFs. Immunofluorescence staining demonstrated that C5aRA inhibited LPS-stimulated nuclear translocation of p-p65. RT-qPCR and Western blotting showed that C5aRA reduced the expression of IL-1β, IL-6, TNF-α, C5aR, p-p65, p-IκBα, p-JNK, p-c-JUN, and TLR4 in LPS-induced HGFs. Conclusion: These findings suggested that C5aRA attenuated the release of inflammatory cytokines in LPS-induced HGFs by blocking the activation of the NF-κB and MAPK signaling pathways. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://www.revistas.usp.br/jaos/article/view/207724/191102; https://www.revistas.usp.br/jaos/article/view/207724 |
| الاتاحة: |
https://www.revistas.usp.br/jaos/article/view/207724 |
| Rights: |
Copyright (c) 2023 Journal of Applied Oral Science ; http://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: |
edsbas.BC0B455F |
| قاعدة البيانات: |
BASE |