Academic Journal
Transcriptional analysis of cystic fibrosis airways at single-cell resolution reveals altered epithelial cell states and composition
| العنوان: | Transcriptional analysis of cystic fibrosis airways at single-cell resolution reveals altered epithelial cell states and composition |
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| المؤلفون: | Carraro, G, Langerman, J, Sabri, S, Lorenzana, Z, Purkayastha, A, Zhang, G, Konda, B, Aros, C.J, Calvert, B.A, Szymaniak, A, Wilson, E, Mulligan, M, Bhatt, P, Lu, J, Vijayaraj, P, Yao, C, Shia, D.W, Lund, A.J, Israely, E, Rickabaugh, T.M, Ernst, J, Mense, M, Randell, S.H, Vladar, E.K, Ryan, A.L, Plath, K, Mahoney, J.E, Stripp, B.R, Gomperts, B.N |
| المصدر: | Nature Medicine, 27(5) |
| بيانات النشر: | Nature Research |
| سنة النشر: | 2021 |
| المجموعة: | Carolina Digital Repository (UNC - University of North Carolina) |
| مصطلحات موضوعية: | respiratory mucosa, Ki 67 antigen, lung transplantation, Cell Differentiation, lrrc6 protein, cytology, trefoil factor 3, Humans, cytokeratin 5, respiratory epithelium, gene expression profiling, cystic fibrosis transmembrane conductance regulator, tumor protein p63, transcriptome, transcriptional regulator rfx3, heat shock protein 27, ankyrin repeat domain protein, pathology, HLA DP antigen, biosynthesis, clinical trial, mucin 5AC, protein, HLA DPA1 antigen, adult, angiogenin, single cell analysis, prrx2 protein, epithelium cell, human cell |
| الوصف: | Cystic fibrosis (CF) is a lethal autosomal recessive disorder that afflicts more than 70,000 people. People with CF experience multi-organ dysfunction resulting from aberrant electrolyte transport across polarized epithelia due to mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. CF-related lung disease is by far the most important determinant of morbidity and mortality. Here we report results from a multi-institute consortium in which single-cell transcriptomics were applied to define disease-related changes by comparing the proximal airway of CF donors (n = 19) undergoing transplantation for end-stage lung disease with that of previously healthy lung donors (n = 19). Disease-dependent differences observed include an overabundance of epithelial cells transitioning to specialized ciliated and secretory cell subsets coupled with an unexpected decrease in cycling basal cells. Our study yields a molecular atlas of the proximal airway epithelium that will provide insights for the development of new targeted therapies for CF airway disease. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://cdr.lib.unc.edu/downloads/g732dm79h?file=thumbnail; https://cdr.lib.unc.edu/downloads/g732dm79h |
| DOI: | 10.17615/st1d-jm14 |
| الاتاحة: | https://doi.org/10.17615/st1d-jm14 https://cdr.lib.unc.edu/downloads/g732dm79h?file=thumbnail https://cdr.lib.unc.edu/downloads/g732dm79h |
| Rights: | http://rightsstatements.org/vocab/InC/1.0/ |
| رقم الانضمام: | edsbas.BBFBACCF |
| قاعدة البيانات: | BASE |
| DOI: | 10.17615/st1d-jm14 |
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