Academic Journal
YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules
| العنوان: | YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules |
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| المؤلفون: | Chernov, Konstantin, Mechulam, Alain, Popova, Nadezhda, PASTRE, David, Nadezhdina, Elena, Skabkina, Olga, Shanina, Nina, Vasiliev, Viktor, Couturier-Tarrade, Anne, Melki, Judith, Joshi, Vandana, Baconnais, Sonia, Toma, Flavio, Ovchinnikov, Lev, Curmi, Patrick |
| المساهمون: | Structure et activité des biomolécules normales et pathologiques (SABNP), Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute of Protein Research, Russian Academy of Sciences, Pushchino, Lomonosov Moscow State University (MSU), Interactions moléculaires et cancer (IMC (UMR 8126)), Signalisation, noyaux et innovations en cancérologie (UMR8126), Centre National de la Recherche Scientifique (CNRS)-Institut Gustave Roussy (IGR)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut Gustave Roussy (IGR)-Université Paris-Sud - Paris 11 (UP11) |
| المصدر: | ISSN: 1471-2091 ; BMC Biochemistry ; https://hal-univ-evry.archives-ouvertes.fr/hal-02166798 ; BMC Biochemistry, BioMed Central, 2008, 9 (1), pp.23. ⟨10.1186/1471-2091-9-23⟩. |
| بيانات النشر: | HAL CCSD BioMed Central |
| سنة النشر: | 2008 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; BACKGROUND: YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs.RESULTS: We show here that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro. High resolution imaging via electron and atomic force microscopy revealed that microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure and indicated that YB-1 most probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Finally, we demonstrated that tubulin interferes with RNA:YB-1 complexes.CONCLUSION: These results suggest that YB-1 may regulate microtubule assembly in vivo and that its interaction with tubulin may contribute to the control of mRNA translation |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-02166798; https://hal-univ-evry.archives-ouvertes.fr/hal-02166798; https://hal-univ-evry.archives-ouvertes.fr/hal-02166798/document; https://hal-univ-evry.archives-ouvertes.fr/hal-02166798/file/%282008%29%20YB-1%20promotes%20microtubule%20assembly%20in%20vitro%20through%20interaction.pdf |
| DOI: | 10.1186/1471-2091-9-23 |
| الاتاحة: | https://hal-univ-evry.archives-ouvertes.fr/hal-02166798 https://hal-univ-evry.archives-ouvertes.fr/hal-02166798/document https://hal-univ-evry.archives-ouvertes.fr/hal-02166798/file/%282008%29%20YB-1%20promotes%20microtubule%20assembly%20in%20vitro%20through%20interaction.pdf https://doi.org/10.1186/1471-2091-9-23 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.BBC23FCC |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/1471-2091-9-23 |
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