التفاصيل البيبلوغرافية
| العنوان: |
Establishment and Phytochemical Analysis of a Callus Culture from Ageratina pichinchensis (Asteraceae) and Its Anti-Inflammatory Activity |
| المؤلفون: |
Mariana Sánchez-Ramos, Silvia Marquina Bahena, Antonio Romero-Estrada, Antonio Bernabé-Antonio, Francisco Cruz-Sosa, Judith Gonzálesssz-Christen, Juan José Acevedo-Fernández, Irene Perea-Arango, Laura Alvarez |
| المصدر: |
Molecules; Volume 23; Issue 6; Pages: 1258 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2018 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
Ageratina pichinchensis, dihydrobenzofuran, 3-epilupeol, callus culture, anti-inflammatory |
| جغرافية الموضوع: |
agris |
| الوصف: |
A protocol was established to produce bioactive compounds in a callus culture of Ageratina pichinchensis by using 1 mg L−1 NAA with 0.1 mg L−1 KIN. The phytochemical study of the EtOAc extract obtained from the callus biomass, allowed the isolation and characterization of eleven secondary metabolites, of which dihydrobenzofuran (5) and 3-epilupeol (7), showed important anti-inflammatory activity. Compound 5 inhibits in vitro the secretion of NO (IC50 = 36.96 ± 1.06 μM), IL-6 (IC50 = 73.71 ± 3.21 μM), and TNF-α (IC50 = 73.20 ± 5.99 μM) in RAW (Murine macrophage cells) 264.7 macrophages, as well as the activation of NF-κB (40% at 150 μM) in RAW-blue macrophages, while compound 7 has been described that inhibit the in vivo TPA-induced ear edema, and the in vitro production of NO, and the PLA2 enzyme activity. In addition, quantitative GC-MS analysis showed that the anti-inflammatory metabolites 5 and 7 were not detected in the wild plant. Overall, our results indicated that A. pichinchensis can be used as an alternative biotechnological resource for obtaining anti-inflammatory compounds. This is the first report of the anti-inflammatory activity of compound 5 and its production in a callus culture of A. pichinchensis. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://dx.doi.org/10.3390/molecules23061258 |
| DOI: |
10.3390/molecules23061258 |
| الاتاحة: |
https://doi.org/10.3390/molecules23061258 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.BBAC23F6 |
| قاعدة البيانات: |
BASE |