Academic Journal
Regenerating the Enthesis: Characterizing Tendon to Bone Interface Development on Polymer Scaffolds Under Cyclic Strain Application
| العنوان: | Regenerating the Enthesis: Characterizing Tendon to Bone Interface Development on Polymer Scaffolds Under Cyclic Strain Application |
|---|---|
| المؤلفون: | Eichaker, Lauren |
| المصدر: | Electronic Theses and Dissertations |
| بيانات النشر: | University of Memphis Digital Commons |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Enthesis, tendon-to-bone interface, bioreactor, cyclic strain, media perfusion |
| الوصف: | This research is the application of cyclic strain to a polyester terepthalate (PET) scaffold seeded with fibroblast cells on one half and osteoblast cells on the other half for 28 days. The apparatus ensured that osteoblasts received no applied strain except that from the flowing media. Fibroblasts received strain (0%, 2.5%, and 5%) in the form of 0.089 Hz load cycles, which incorporated 10 seconds of rest, 4 hours per day. This preliminary research showed that fibroblasts can respond to strain application and media flow by depositing approximately 2 times more glycosaminoglycans and collagen matrix onto the PET scaffold per cell (μg/μg) in comparison to a static condition. In addition, osteoblast cells produced osteocalcin, signifying that the bioreactor environment that incorporated media flow and/or cyclic strain to the fibroblasts promoted osteoblast maturation. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | https://digitalcommons.memphis.edu/etd/776; https://digitalcommons.memphis.edu/context/etd/article/1884/viewcontent/Masters_Thesis_Draft_Final.pdf |
| الاتاحة: | https://digitalcommons.memphis.edu/etd/776 https://digitalcommons.memphis.edu/context/etd/article/1884/viewcontent/Masters_Thesis_Draft_Final.pdf |
| رقم الانضمام: | edsbas.BB84D4FF |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |