التفاصيل البيبلوغرافية
| العنوان: |
Determination of Morphine and Its Metabolites in Human Urine by Capillary Electrophoresis with Laser Induced Fluorescence Detection Employing On-Column Labeling with a New Boronic Acid Functionalized Squarylium Cyanine Dye |
| المؤلفون: |
Mahmoud Sebaiy, Abdullah El-Shanawany, Mohamed Baraka, Lobna Abdel-Aziz, Theresa Isbell, Christa Colyer |
| المصدر: |
Separations; Volume 3; Issue 1; Pages: 1 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2016 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
morphine, morphine-3-beta- d -glucuronide, morphine-6-beta- d -glucuronide, boronic acid, squarylium dye, on-column labeling, fluorescence, capillary electrophoresis |
| الوصف: |
A novel method for the labeling and rapid separation of morphine, morphine-3-beta-d-glucuronide (M3G) and morphine-6-beta-d-glucuronide (M6G) in human urine employing a new boronic acid functionalized squarylium dye (SQ-BA3) and capillary electrophoresis with laser induced fluorescence detection (CE-LIF) is described. The spectrochemical properties, solution stability, pH range, and mechanisms for interactions with morphine and its metabolites were first established for SQ-BA3, followed by optimization of an on-column labeling procedure and CE-LIF method. SQ-BA3 itself was shown to be unstable and weakly fluorescent in aqueous buffers due to aggregate formation. However, SQ-BA3 showed a relative stability and dramatic increase in fluorescence intensity upon the addition of morphine, M3G, and M6G. Because of the low background fluorescence of this dye, on-column labeling was feasible, leading to a simple and rapid analytical method with the potential for clinical applications. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://dx.doi.org/10.3390/chromatography3010001 |
| DOI: |
10.3390/chromatography3010001 |
| الاتاحة: |
https://doi.org/10.3390/chromatography3010001 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.BB06C47F |
| قاعدة البيانات: |
BASE |