Academic Journal
Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters
| العنوان: | Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters |
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| المؤلفون: | Robles-Rebollo, Irene, Cuartero, Sergi, Canellas-Socias, Adria, Wells, Sarah, Karimi, Mohammad M., Mereu, Elisabetta, Chivu, Alexandra G., Heyn, Holger, Whilding, Chad, Dormann, Dirk, Marguerat, Samuel, Rioja, Inmaculada, Prinjha, Rab K., Stumpf, Michael P.H., Fisher, Amanda G., Merkenschlager, Matthias |
| بيانات النشر: | Nature Research |
| سنة النشر: | 2022 |
| المجموعة: | UPF Digital Repository (Universitat Pompeu Fabra, Barcelona) |
| مصطلحات موضوعية: | Nuclear organization, Transcription |
| الوصف: | Innate immune responses rely on inducible gene expression programmes which, in contrast to steady-state transcription, are highly dependent on cohesin. Here we address transcriptional parameters underlying this cohesin-dependence by single-molecule RNA-FISH and single-cell RNA-sequencing. We show that inducible innate immune genes are regulated predominantly by an increase in the probability of active transcription, and that probabilities of enhancer and promoter transcription are coordinated. Cohesin has no major impact on the fraction of transcribed inducible enhancers, or the number of mature mRNAs produced per transcribing cell. Cohesin is, however, required for coupling the probabilities of enhancer and promoter transcription. Enhancer-promoter coupling may not be explained by spatial proximity alone, and at the model locus Il12b can be disrupted by selective inhibition of the cohesinopathy-associated BET bromodomain BD2. Our data identify discrete steps in enhancer-mediated inducible gene expression that differ in cohesin-dependence, and suggest that cohesin and BD2 may act on shared pathways. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | Nat Commun. 2022 Jul 27;13(1):4342; http://hdl.handle.net/10230/54693 |
| DOI: | 10.1038/s41467-022-31192-9 |
| الاتاحة: | http://hdl.handle.net/10230/54693 https://doi.org/10.1038/s41467-022-31192-9 |
| Rights: | © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. ; http://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.BAFB91CF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-022-31192-9 |
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