Academic Journal
Quantitative analysis of lignocaine and metabolites in equine urine and plasma by liquid chromatography-tandem mass spectrometry
| العنوان: | Quantitative analysis of lignocaine and metabolites in equine urine and plasma by liquid chromatography-tandem mass spectrometry |
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| المؤلفون: | Nelis, Samantha A., Sievers, Catherine, Jarrett, Mark, Nissen, Lisa M., Kirkpatrick, Carl M. J., Shaw, P. Nicholas |
| المساهمون: | R. P. H. Bischoff, D. S. Hage, G. Hopfgartner, H. T. Karnes, D. K. Lloyd, P. J. Taylor, G. Xu |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2010 |
| المجموعة: | The University of Queensland: UQ eSpace |
| مصطلحات موضوعية: | Lignocaine, Lidocaine, Horse, LCMS, Liquid chromatography, Mass spectrometry, 2700 Medicine |
| الوصف: | In this paper, a method for the sensitive and reproducible analysis of lignocaine and its four principal metabolites, monoethylxylidide (MEGX), glycylxylidide (GX), 3-hydroxylignocaine (3-HO-LIG), 4-hydroxylignocaine (4-HO-LIG) in equine urine and plasma samples is presented. The method uses liquid chromatography coupled to tandem mass spectrometry operating in electrospray ionisation positive ion mode (+ESI) via multiple reaction monitoring (MRM). Sample preparation involved solid-phase extraction using a mixed-mode phase. The internal standard adopted was lignocaine-d(10). Lignocaine and its metabolites were successfully resolved using an octadecylsilica reversed-phase column using a gradient mobile phase of acetonitrile and 0.1% (v/v) aqueous formic acid at a flow rate of 300 mu L/min. Target analytes and the internal standard were determined by using the following transitions; lignocaine, 235.2 > 86.1; 3-HO-LIG and 4-HO-LIG, 251.2 > 86.1; MEGX, 207.1 > 58.1; GX, 179.1 > 122.1; and lignocaine-d10, 245.2 > 96.1. Calibration curves were generated over the range 1-100 ng/mL for plasma samples and 1-1000 ng/mL for urine samples. The method was validated for instrument linearity, repeatability and detection limit (IDL), method linearity, repeatability, detection limit (MDL), quantitation limit (LOQ) and recovery. The method was successfully used to analyse both plasma and urine samples following a subcutaneous administration of lignocaine to a thoroughbred horse. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1570-0232 1873-376X 1387-2273 |
| Relation: | orcid:0000-0002-6121-8148; orcid:0000-0001-5826-4605; orcid:0000-0001-7154-592X |
| الاتاحة: | https://espace.library.uq.edu.au/view/UQ:211392 |
| رقم الانضمام: | edsbas.BA7DA4E2 |
| قاعدة البيانات: | BASE |
| تدمد: | 15700232 1873376X 13872273 |
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