Academic Journal
3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model
| العنوان: | 3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model |
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| المؤلفون: | Lukas Otto, Petra Wolint, Annina Bopp, Anna Woloszyk, Anton S. Becker, Andreas Boss, Roland Böni, Maurizio Calcagni, Pietro Giovanoli, Simon P. Hoerstrup, Maximilian Y. Emmert, Johanna Buschmann |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
| بيانات النشر: | Nature Portfolio |
| سنة النشر: | 2021 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Bone regeneration is a complex process and the clinical translation of tissue engineered constructs (TECs) remains a challenge. The combination of biomaterials and mesenchymal stem cells (MSCs) may enhance the healing process through paracrine effects. Here, we investigated the influence of cell format in combination with a collagen scaffold on key factors in bone healing process, such as mineralization, cell infiltration, vascularization, and ECM production. MSCs as single cells (2D-SCs), assembled into microtissues (3D-MTs) or their corresponding secretomes were combined with a collagen scaffold and incubated on the chicken embryo chorioallantoic membrane (CAM) for 7 days. A comprehensive quantitative analysis was performed on a cellular level by histology and by microcomputed tomography (microCT). In all experimental groups, accumulation of collagen and glycosaminoglycan within the scaffold was observed over time. A pronounced cell infiltration and vascularization from the interface to the surface region of the CAM was detected. The 3D-MT secretome showed a significant mineralization of the biomaterial using microCT compared to all other conditions. Furthermore, it revealed a homogeneous distribution pattern of mineralization deposits in contrast to the cell-based scaffolds, where mineralization was only at the surface. Therefore, the secretome of MSCs assembled into 3D-MTs may represent an interesting therapeutic strategy for a next-generation bone healing concept. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doi.org/10.1038/s41598-021-84123-x; https://doaj.org/toc/2045-2322; https://doaj.org/article/13a7a4e40eaf4c73bc17ec0a47a77cc5 |
| DOI: | 10.1038/s41598-021-84123-x |
| الاتاحة: | https://doi.org/10.1038/s41598-021-84123-x https://doaj.org/article/13a7a4e40eaf4c73bc17ec0a47a77cc5 |
| رقم الانضمام: | edsbas.B9F9A2FC |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-021-84123-x |