Academic Journal
Caspase 3 Activity and Lipoperoxidative Status in Raw Semen Predict the Outcome of Cryopreservation of Stallion Spermatozoa
| العنوان: | Caspase 3 Activity and Lipoperoxidative Status in Raw Semen Predict the Outcome of Cryopreservation of Stallion Spermatozoa |
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| المؤلفون: | Martín Muñoz, Patricia, Ortega Ferrusola, Cristina, Anel López, Luis, del Petre, Chiara, Álvarez García, Mercedes, Paz Cabello, Paulino de, Anel Rodríguez, Luis, Peña Vega, Fernando Juan |
| المساهمون: | Sanidad Animal, Facultad de Veterinaria |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2016 |
| المجموعة: | Universidad de León: BULERIA |
| مصطلحات موضوعية: | Veterinaria, 4-HNE, 8-ISO-PGF, 8-OH guanosine, Cryopreservation, Equids, Flow cytometry, ROS, Sperm, Stallion |
| الوصف: | [EN] Stallion-to-stallion variability in the quality of cryopreserved ejaculates postthaw affects the commercial acceptability of frozen semen and thus is a major constraint for the equine industry. In recent years, the molecular mechanisms associated with sperm damage during cryopreservation have become better understood. Identification of the freezability of the ejaculates before the freezing process is initiated will have a major impact on the equine industry. We studied three markers of oxidative stress in sperm, including 8-iso-PGF2alpha, 8-OH guanosine, and 4-hydroxynonenal (4-HNE); the presence of active caspase 3; and their changes after sperm cryopreservation. Although 4- HNE levels increased after cryopreservation (from 7% to 33%, P < 0.001), 8OH-guanosine and 8-ISO-PGF2alpha levels decreased after cryopreservation (from 130 to 35 arbitrary fluorescence units, P < 0.01, and from 1280 to 1233, P < 0.01, respectively). Postthaw sperm quality was classified as poor, average, or good using the 25th and 75th percentiles of all assays of sperm quality studied (motility, velocity, membrane functionality, and thiol content) as thresholds. Using these values, a sperm postthaw quality index was proposed. Receiver operating characteristic curves and the Youden J statistic were used to investigate the value of the measured parameters in fresh sperm as predictors of potential freezability. Using these techniques, we identified markers of bad freezers (percentages of caspase 3-positive dead sperm [area under the curve (AUC)= 0.820, P < 0.05] and percentages of caspase 3- and 4-HNEpositive sperm [AUC = 0.872, P < 0.05]) and good freezers (percentages of caspase 3-negative live sperm [AUC = 0.815, P < 0.05], percentages of live sperm with high thiol content [AUC = 0.907, P < 0.01], and percentages of 8-ISO-PGF2alphapositive sperm [AUC = 0.900, P < 0.01]. Moreover, we described for the first time the presence of 8-ISO-PGF2alpha in stallion spermatozoa and revealed the importance of considering ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0006-3363 1529-7268 |
| Relation: | Muñoz, P. M., Ferrusola, C. O., Lopez, L. A., Del Petre, C., Garcia, M. A., Cabello, P. d. P., Anel, L., & Peña, F. J. (2016). Caspase 3 activity and lipoperoxidative status in raw semen predict the outcome of cryopreservation of stallion spermatozoa. Biology of Reproduction, 95(3). https://doi.org/10.1095/BIOLREPROD.116.139444; https://academic.oup.com/biolreprod/article/95/3/53,%201-12/2883474?login=true; https://hdl.handle.net/10612/18513 |
| DOI: | 10.1095/BIOLREPROD.116.139444 |
| الاتاحة: | https://hdl.handle.net/10612/18513 https://academic.oup.com/biolreprod/article/95/3/53,%201-12/2883474?login=true https://doi.org/10.1095/BIOLREPROD.116.139444 |
| Rights: | Attribution-NoDerivatives 4.0 Internacional ; http://creativecommons.org/licenses/by-nd/4.0/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.B9D480E4 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00063363 15297268 |
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| DOI: | 10.1095/BIOLREPROD.116.139444 |