Academic Journal
Mitochondrial genome copy number variation across tissues in mice and humans
| العنوان: | Mitochondrial genome copy number variation across tissues in mice and humans |
|---|---|
| المؤلفون: | Rath, Sneha P., Gupta, Rahul, Todres, Ellen, Wang, Hong, Jourdain, Alexis A., Ardlie, Kristin G., Calvo, Sarah E., Mootha, Vamsi K. |
| المصدر: | Proceedings of the National Academy of Sciences, vol. 121, no. 33 |
| سنة النشر: | 2024 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| الوصف: | The mammalian mitochondrial genome (mtDNA) is multicopy and its copy number (mtCN) varies widely across tissues, in development and in disease. Here, we system- atically catalog this variation by assaying mtCN in 52 human tissues across 952 donors (10,499 samples from the Genotype-Tissue Expression project) and 20 murine tissues using qPCR, capturing 50- and 200-fold variation, respectively. We also estimate per cell mtCN across 173 human cell lines from the Cancer Cell Line Encyclopedia using whole- genome sequencing data and observe >50- fold variation. We then leverage the vast amount of genomics data available for these repositories to credential our resource and uncover mtDNA-related biology. Using already existing proteomics data, we show that variation in mtCN can be predicted by variation in TFAM, histone, and mito- chondrial ribosome protein abundance. We also integrate mtCN estimates with the CRISPR gene dependency measurements to find that cell lines with high mtCN are resistant to loss of GPX4, a glutathione phospholipid hydroperoxidase. Our resource captures variation in mtCN across mammalian tissues and should be broadly useful to the research community. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pissn/0027-8424; info:eu-repo/semantics/altIdentifier/pissn/1091-6490; info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_CDDA57E985744; https://serval.unil.ch/notice/serval:BIB_CDDA57E98574; https://serval.unil.ch/resource/serval:BIB_CDDA57E98574.P001/REF.pdf |
| DOI: | 10.1073/pnas.2402291121 |
| الاتاحة: | https://serval.unil.ch/notice/serval:BIB_CDDA57E98574 https://doi.org/10.1073/pnas.2402291121 https://serval.unil.ch/resource/serval:BIB_CDDA57E98574.P001/REF.pdf http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_CDDA57E985744 |
| Rights: | info:eu-repo/semantics/openAccess ; CC BY 4.0 ; https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.B944B98 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1073/pnas.2402291121 |
|---|