Report
Communication between DNA polymerases and Replication Protein A within the archaeal replisome
| العنوان: | Communication between DNA polymerases and Replication Protein A within the archaeal replisome |
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| المؤلفون: | Martínez-Carranza, Markel, Vialle, Léa, Madru, Clément, Cordier, Florence, Tekpinar, Ayten, Dizkirici, Haouz, Ahmed, Legrand, Pierre, Le Meur, Rémy, A, England, Patrick, Dulermo, Rémi, Guijarro, J. I., Henneke, Ghislaine, Sauguet, Ludovic |
| المساهمون: | Architecture et Dynamique des Macromolécules Biologiques - Architecture and Dynamics of Biological Macromolecules, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Biologie et Ecologie des Ecosystèmes Marins Profonds (BEEP), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Plateforme Technologique de RMN Biologique et HDX-MS - Biological NMR and HDX-MS Technological Platform, Bioinformatique structurale - Structural Bioinformatics, Cristallographie (Plateforme) - Crystallography (Platform), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Biophysique Moléculaire (plateforme) - Molecular Biophysics (platform), We gratefully acknowledge the financial support of ANR (Grant ARCHAPRIM). C.M. was funded by a postdoctoral Pasteur-Roux-Cantarini fellowship. M.M.C. was funded by apostdoctoral FRM fellowship (Fondation pour la Recherche Médicale). A.D.T. was founded by a Pause grant from Collège de France. We wish to acknowledge SOLEIL for the provision of synchrotron radiation facilities and the staff of beamlines PROXIMA-1 and PROXIMA-2 (Saint-Aubin, France). We would like to thank the staff of the Nanoimaging Core Facility at Institut Pasteur for cryo-EM instrument access for the preliminary cryo-EM datasets. Electron microscopy instrument time was provided through the OPIC electron microscopy facility, a UK Instruct-ERIC Centre, which was founded by a Wellcome JIF award (060208/Z/00/Z) and is supported by a Wellcome equipment grant (093305/Z/10/Z). Cryo-EM final data collection experiments were funded by UK Instruct-ERIC, PID: 21658. The 800-MHz NMR spectrometer of the Institut Pasteur was partially funded by the Région Ile de France (SESAME 2014 NMRCHR grant no 4014526)., ANR-20-CE11-0003,ARCHAPRIM,L'ADN primase, le couteau Suisse des Archées: Etude de biologie intégrative(2020) |
| المصدر: | https://hal.science/hal-04728470 ; 2024. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | Replication Protein A (RPA) plays a pivotal role in DNA replication by coating and protecting exposed single-stranded DNA, and acting as a molecular hub that recruits additional replication factors. We demonstrated that archaeal RPA hosts a winged-helix domain (WH) that interacts with two key actors of the replisome: the DNA primase (PriSL) and the replicative DNA polymerase (PolD). Using an integrative structural biology approach, combining nuclear magnetic resonance, X-ray crystallography and cryo-electron microscopy, we unveiled how RPA interacts with PriSL and PolD through two distinct surfaces of the WH domain: an evolutionarily conserved interface and a novel binding site. Finally, RPA was shown to stimulate the activity of PriSL in a WH-dependent manner. This study provides a molecular understanding of the WH-mediated regulatory activity in conserved central replication factors such as RPA, which regulate genome maintenance in Archaea and Eukaryotes. |
| نوع الوثيقة: | report |
| اللغة: | English |
| Relation: | BIORXIV: 2024.04.29.591592 |
| DOI: | 10.1101/2024.04.29.591592 |
| الاتاحة: | https://hal.science/hal-04728470 https://hal.science/hal-04728470v1/document https://hal.science/hal-04728470v1/file/2024.04.29.591592v1.full.pdf https://doi.org/10.1101/2024.04.29.591592 |
| Rights: | http://hal.archives-ouvertes.fr/licences/copyright/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.B8ECF1BB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2024.04.29.591592 |
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