Academic Journal
NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration
| العنوان: | NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration |
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| المؤلفون: | Laplace-Builhé, Béryl, Barthelaix, Audrey, Assou, Said, Bohaud, Candice, Pratlong, Marine, Severac, Dany, Tejedor, Gautier, Luz-Crawford, Patricia, Nguyen-Chi, Mai, Mathieu, Marc, Jorgensen, Christian, Djouad, Farida |
| المساهمون: | Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), BioCampus (BCM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Universidad de los Andes Santiago (UANDES), Laboratory of Pathogen Host Interactions Montpellier (LPHI), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire Montpellier (CHRU Montpellier), ANR-10-INBS-0009,France Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010) |
| المصدر: | ISSN: 2041-1723. |
| بيانات النشر: | HAL CCSD Nature Publishing Group |
| سنة النشر: | 2021 |
| المجموعة: | Université de Montpellier: HAL |
| مصطلحات موضوعية: | MESH: Animal Fins, MESH: Animals, MESH: Zebrafish, MESH: Zebrafish Proteins, MESH: Cell Proliferation, MESH: Forkhead Transcription Factors, MESH: Gene Expression Regulation, Developmental, MESH: Genes, erbB, MESH: Larva, MESH: Macrophages, MESH: Neural Crest, MESH: Neuregulin-1, MESH: Regeneration, MESH: Signal Transduction, MESH: Stem Cells, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] |
| الوصف: | International audience ; Fish species, such as zebrafish ( Danio rerio ), can regenerate their appendages after amputation through the formation of a heterogeneous cellular structure named blastema. Here, by combining live imaging of triple transgenic zebrafish embryos and single-cell RNA sequencing we established a detailed cell atlas of the regenerating caudal fin in zebrafish larvae. We confirmed the presence of macrophage subsets that govern zebrafish fin regeneration, and identified a foxd3 -positive cell population within the regenerating fin. Genetic depletion of these foxd3 -positive neural crest-derived cells (NCdC) showed that they are involved in blastema formation and caudal fin regeneration. Finally, chemical inhibition and transcriptomic analysis demonstrated that these foxd3 -positive cells regulate macrophage recruitment and polarization through the NRG1/ErbB pathway. Here, we show the diversity of the cells required for blastema formation, identify a discrete foxd3 -positive NCdC population, and reveal the critical function of the NRG1/ErbB pathway in controlling the dialogue between macrophages and NCdC. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/34732706; PUBMED: 34732706; PUBMEDCENTRAL: PMC8566576 |
| DOI: | 10.1038/s41467-021-26422-5 |
| الاتاحة: | https://hal.umontpellier.fr/hal-03646943 https://hal.umontpellier.fr/hal-03646943v1/document https://hal.umontpellier.fr/hal-03646943v1/file/s41467-021-26422-5.pdf https://doi.org/10.1038/s41467-021-26422-5 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.B8EAE584 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-021-26422-5 |
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