Academic Journal
Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin
| العنوان: | Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin |
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| المؤلفون: | Tarantini, Francesco Saverio, Brunati, Mara, Taravella, Anna, Carrano, Lucia, Parenti, Francesco, Hong, Kar Wai, Williams, Paul, Chan, Kok Gan, Heeb, Stephan, Chan, Weng C. |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2021 |
| المجموعة: | University of Nottingham: Repository@Nottingham |
| مصطلحات موضوعية: | Multidisciplinary |
| الوصف: | As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the most similar characterised species. However, whole-genome sequencing revealed an average nucleotide identity (ANI) value of 89% with A. macra, the highest among related species. Further phenotypic and chemotaxonomic analyses confirmed that the isolate represents a previously uncharacterised species in the genus Actinomadura, for which the name Actinomadura graeca sp. nov. is proposed (type strain 32-07T). The G+C content of A. graeca 32-07 is 72.36%. The cell wall contains DL-diaminopimelic acid, intracellular sugars are glucose, ribose and galactose, the predominant menaquinone is MK-9(H6), the major cellular lipid is phosphatidylinositol and fatty acids consist mainly of hexadecanoic acid. No mycolic acid was detected. Furthermore, A. graeca 32-07 has been confirmed as a novel producer of the non-ribosomal peptide antibiotic zelkovamycin and we report herein a provisional description of the unique biosynthetic gene cluster. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://nottingham-repository.worktribe.com/output/6842832; PLoS ONE; Volume 16; Issue 11 |
| DOI: | 10.1371/journal.pone.0260413 |
| الاتاحة: | https://doi.org/10.1371/journal.pone.0260413 https://nottingham-repository.worktribe.com/file/6842832/1/Actinomadura%20graeca%20sp.%20nov.%3A%20A%20novel%20producer%20of%20the%20macrocyclic%20antibiotic%20zelkovamycin https://nottingham-repository.worktribe.com/output/6842832 |
| Rights: | openAccess ; https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.B8E69A59 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0260413 |
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