Academic Journal
Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake
| العنوان: | Applying TADF Emitters in Bioimaging and Sensing—A Novel Approach Using Liposomes for Encapsulation and Cellular Uptake |
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| المؤلفون: | Smith, Poppy O., Black, Dominic J., Pal, Robert, Avó, João, Dias, Fernando B., Linthwaite, Victoria L., Cann, Martin J., Pålsson, Lars-Olof |
| المصدر: | Frontiers in chemistry, 2021, Vol.9, pp.743928 [Peer Reviewed Journal] |
| بيانات النشر: | Frontiers Media |
| سنة النشر: | 2021 |
| المجموعة: | Durham University: Durham Research Online |
| الوصف: | A new method for facilitating the delivery, uptake and intracellular localisation of thermally activated delayed fluorescence (TADF) complexes was developed. First, confinement of TADF complexes in liposomes was demonstrated, which were subsequently used as the delivery vehicle for cellular uptake. Confocal fluorescence microscopy showed TADF complexes subsequently localise in the cytoplasm of HepG2 cells. The procedures developed in this work included the removal of molecular oxygen in the liposome preparation without disrupting the liposome structures. Time-resolved fluorescence microscopy (point scanning) showed initial prompt fluorescence followed by a weak, but detectable, delayed fluorescence component for liposomal TADF internalised in HepG2 cells. By demonstrating that it is possible to deliver un-functionalised and/or unshielded TADF complexes, a sensing function for TADFs, such as molecular oxygen, can be envisaged. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| تدمد: | 2296-2646 |
| Relation: | dro:34140; http://dro.dur.ac.uk/34140/; https://doi.org/10.3389/fchem.2021.743928; http://dro.dur.ac.uk/34140/1/34140.pdf |
| DOI: | 10.3389/fchem.2021.743928 |
| الاتاحة: | http://dro.dur.ac.uk/34140/ http://dro.dur.ac.uk/34140/1/34140.pdf https://doi.org/10.3389/fchem.2021.743928 |
| Rights: | © 2021 Smith, Black, Pal, Avó, Dias, Linthwaite, Cann and Pålsson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| رقم الانضمام: | edsbas.B88887FF |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 22962646 |
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| DOI: | 10.3389/fchem.2021.743928 |