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Probing sulfatide-tissue lectin recognition with functionalized glycodendrimersomes

التفاصيل البيبلوغرافية
العنوان: Probing sulfatide-tissue lectin recognition with functionalized glycodendrimersomes
المؤلفون: Murphy, Paul V., Romero, Antonio, Xiao, Qi, Ludwig, Anna-Kristin, Jogula, Srinivas, Shilova, Nadezhda V., Singh, T., Gabba, Adele, Javed, Bilal, Zhang, Dapeng, Medrano, Francisco Javier, Kaltner, Herbert, Kopitz, Jürgen, Bovin, Nicolai V., Wu, Albert M., Klein, Michael L., Percec, V., Gabius, Hans-Joachim
المساهمون: National Science Foundation (US), European Commission, Ministerio de Economía y Competitividad (España), European Cooperation in Science and Technology, Romero, Antonio, Xiao, Qi, Ludwig, Anna-Kristin, Jogula, Srinivas, Gabba, Adele, Javed, Bilal, Zhang, Dapeng, Medrano, Francisco Javier, Kaltner, Herbert, Kopitz, Jürgen, Percec, V., Gabius, Hans-Joachim
بيانات النشر: Cell Press
سنة النشر: 2021
المجموعة: Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council)
الوصف: 74 p.-5 fig.-3 schem.-6 fig. supl.-4 tab.supl.-3 schem. supl ; The small 3-O-sulfated galactose head group of sulfatides, an abundant glycosphingolipid class, poses the (sphinx-like) riddle on involvement of glycan bridging by tissue lectins (sugar code). First, synthesis of head group derivatives for functionalization of amphiphilic dendrimers is performed. Aggregation of resulting (biomimetic) vesicles, alone or in combination with lactose, demonstrates bridging by a tissue lectin (galectin-4). Physiologically, this can stabilize glycolipid-rich microdomains (rafts) and associate sulfatide-rich regions with specific glycoproteins. Further testing documents importance of heterobivalency and linker length. Structurally, sulfatide recognition by galectin-8 is shown to involve sphingosine’s OH group as substitute for the 30-hydroxyl of glucose of lactose.These discoveries underscore functionality of this small determinant on biomembranes intracellularly and on the cell surface. Moreover, they provide a role model to examine counterreceptor capacity of more complex glycans of glycosphingolipids and to start their bottom-up glycotope surface programming. ; This work is supported by NSF Grants DMR1066116, DMR-1720530, and DMR-1807127 (to V.P.), the P. Roy Vagelos Chair at the University of Pennsylvania (V.P.), the Sheikh Saqr Research Foundation (to M.L.K.), the Science Foundation Ireland (SFI) and the European Regional Development Fund (Grant Number 13/RC/ 2073 to CÚRAM, 16/IA/4419 to P.V.M.), the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 713690 (Medtrain, to CÚRAM & S.J.), the Irish Research Council (PhD scholarship to A.G.), the Grant BFU2016-77835-R of the Spanish Ministry of Economy and Competitiveness (A.R.), as well as the COST Action CA18103 (InnoGly). ; Peer reviewed
نوع الوثيقة: article in journal/newspaper
اللغة: English
تدمد: 2589-0042
Relation: #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/EC/H2020/713690; info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-77835-R; Publisher's version; https://doi.org/10.1016/j.isci.2020.101919; Sí; iScience 24:101919 (2021); http://hdl.handle.net/10261/229465; http://dx.doi.org/10.13039/501100003329; http://dx.doi.org/10.13039/100000001; http://dx.doi.org/10.13039/501100000780; http://dx.doi.org/10.13039/501100000921
DOI: 10.1016/j.isci.2020.101919
DOI: 10.13039/501100003329
DOI: 10.13039/100000001
DOI: 10.13039/501100000780
DOI: 10.13039/501100000921
الاتاحة: http://hdl.handle.net/10261/229465
https://doi.org/10.1016/j.isci.2020.101919
https://doi.org/10.13039/501100003329
https://doi.org/10.13039/100000001
https://doi.org/10.13039/501100000780
https://doi.org/10.13039/501100000921
Rights: open
رقم الانضمام: edsbas.B841FAEA
قاعدة البيانات: BASE
الوصف
تدمد:25890042
DOI:10.1016/j.isci.2020.101919