Academic Journal
The essential role of mRNA degradation in understanding and engineering E. coli metabolism
| العنوان: | The essential role of mRNA degradation in understanding and engineering E. coli metabolism |
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| المؤلفون: | Roux, Charlotte, Etienne, Thibault, Hajnsdorf, Eliane, Ropers, Delphine, Carpousis, Agamemnon, J., Cocaign-Bousquet, Muriel, Girbal, Laurence |
| المساهمون: | Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de biologie physico-chimique (IBPC (FR_550)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Analyse, ingénierie et contrôle des micro-organismes (MICROCOSME), Inria Grenoble - Rhône-Alpes, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Université Grenoble Alpes (UGA), Laboratoire de microbiologie et génétique moléculaires - UMR5100 (LMGM), Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the French National Research Agency ANR-18-CE43-0010 ., ANR-18-CE43-0010,RIB-ECO,Ingénierie du cycle de vie des ARN pour une approche économique de l'énergétique microbienne: application à la bioconversion des sources de carbone dérivées de la biomasse(2018) |
| المصدر: | ISSN: 0734-9750 ; Biotechnology Advances ; https://hal.science/hal-03325744 ; Biotechnology Advances, 2022, pp.1-13. ⟨10.1016/j.biotechadv.2021.107805⟩. |
| بيانات النشر: | CCSD Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | Université Toulouse III - Paul Sabatier: HAL-UPS |
| مصطلحات موضوعية: | Heterologous protein production, Microbe engineering, Biotechnology, mRNA degradation, Metabolism, Systems biology, Gene expression, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Metabolic engineering strategies are crucial for the development of bacterial cell factories with improved performance. Until now, optimal metabolic networks have been designed based on systems biology approaches integrating large-scale data on the steady-state concentrations of mRNA, protein and metabolites, sometimes with dynamic data on fluxes, but rarely with any information on mRNA degradation. In this review, we compile growing evidence that mRNA degradation is a key regulatory level in E. coli that metabolic engineering strategies should take into account. We first discuss how mRNA degradation interacts with transcription and translation, two other gene expression processes, to balance transcription regulation and remove poorly translated mRNAs. The many reciprocal interactions between mRNA degradation and metabolism are also highlighted: metabolic activity can be controlled by changes in mRNA degradation and in return, the activity of the mRNA degradation machinery is controlled by metabolic factors. The mathematical models of the crosstalk between mRNA degradation dynamics and other cellular processes are presented and discussed with a view towards novel mRNA degradation-based metabolic engineering strategies. We show finally that mRNAdegradation-based strategies have already successfully been applied to improve heterologous protein synthesis. Overall, this review underlines how important mRNA degradation is in regulating E. coli metabolism and identifies mRNA degradation as a key target for innovative metabolic engineering strategies in biotechnology. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/34302931; PUBMED: 34302931; WOS: 000820188000009 |
| DOI: | 10.1016/j.biotechadv.2021.107805 |
| الاتاحة: | https://hal.science/hal-03325744 https://hal.science/hal-03325744v1/document https://hal.science/hal-03325744v1/file/1-s2.0-S0734975021001117-main.pdf https://doi.org/10.1016/j.biotechadv.2021.107805 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.B81A1232 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.biotechadv.2021.107805 |
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