Academic Journal
Extract of Calvatia gigantea inhibits proliferation of A549 human lung cancer cells
| العنوان: | Extract of Calvatia gigantea inhibits proliferation of A549 human lung cancer cells |
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| المؤلفون: | EroÄŸlu, Canan, Atmaca, Pelin, Gezer, Kudret, Kaygusuz, OÄŸuzhan, BaÄŸcı, Gülseren, Seçme, Mücahit, Dodurga, Yavuz |
| بيانات النشر: | Springer Netherlands |
| سنة النشر: | 2016 |
| المجموعة: | Pamukkale University Repository / Pamukkale Üniversitesi Açık Erişim Arşivi |
| مصطلحات موضوعية: | Calvatia gigantea, Apoptosis, human, Cell cycle, Lung cancer cell line, protein expression, 2 3 bis (2 methoxy 4 nitro 5 sulfophenyl) 2h tetrazolium 5 carboxanilide, antineoplastic agent, Calvatia gigantea extract, caspase 3, caspase 9, cyclin D1, cyclin D2, cyclin dependent kinase 4, plant extract, protein Bax, protein bcl 2, protein kinase B, protein p21, protein p53, tetrazole derivative, unclassified drug, A549 cell line, antineoplastic activity, antiproliferative activity, Article, cell cycle arrest, cell proliferation, cell viability, controlled study |
| الوصف: | In this study, in order to investigate the anticancer mechanism of Calvatia gigantea extract, edible mushroom species, which belong to Lycoperdaceae family, changes of CCND1, CCND2, CDK4, p21, Akt, Bax, Bcl-2, p53, caspase-3 and caspase-9 were evaluated in A549 lung cancer cells. Cytotoxic effect of C.gigantea extract was evaluated by using XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5 carboxanilide). The C. gigantea extract was treated in a time and dose dependent manner within the range 25 µg/ml–2 mg/ml to determine the IC50 dose. IC50 dose for C. gigantea extract was detected as 500 µg/ml for 72 h. According to expression results, while CCND1, CCND2, CDK4, Akt and Bcl-2 expression clearly decreased, Bax, p53, caspase-3 and caspase-9 expression clearly increased in the dose group cells (A549 cells treated with 500 µg/ml dose of C. gigantea extract for 72 h). However, there was no change in p21 expression. C. gigantea extract induced cell cycle arrest and apoptosis by decreasing the CCND1, CCND2, CDK4, Akt and Bcl-2 expression and by increasing Bax, p53, caspase-3 and caspase-9 expression in A549 cells. Mushrooms are eukaryotic organisms heavily used because of their supposedly anticancer effect. Many mushroom species have been used for medical purposes, as a result of also having many effects such as antibiotic, antiviral and anticancer effects. It is thought that the C. gigantea extract may be a significant agent for treatment of lung cancer as a single agent or in combination with other drugs. © 2016, Springer Science+Business Media Dordrecht. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | Cytotechnology; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://hdl.handle.net/11499/9414; 68; 2075; 2081; WOS:000383495000033 |
| DOI: | 10.1007/s10616-016-9947-4 |
| الاتاحة: | https://hdl.handle.net/11499/9414 https://doi.org/10.1007/s10616-016-9947-4 |
| Rights: | open |
| رقم الانضمام: | edsbas.B7FEAAB8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s10616-016-9947-4 |
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