Academic Journal
The production of (R)-2-Hydroxy-1-phenyl-propan-1-one derivatives by Benzaldehyde lyase from Pseudomonas fluorescens in a continuously operated membrane reactor
| العنوان: | The production of (R)-2-Hydroxy-1-phenyl-propan-1-one derivatives by Benzaldehyde lyase from Pseudomonas fluorescens in a continuously operated membrane reactor |
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| المؤلفون: | Hildebrand, F., Kühl, S., Pohl, M., Vasic-Racki, D., Müller, M., Wandrey, C., Lütz, S. |
| المصدر: | Biotechnology & bioengineering 96, 835 - 843 (2007). doi:10.1002/bit.21189 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2007 |
| المجموعة: | Forschungszentrum Jülich: JuSER (Juelich Shared Electronic Resources) |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/570, Acetone: analogs & derivatives, Acetone: metabolism, Aldehyde-Lyases: metabolism, Bioreactors: microbiology, Membranes: metabolism, Membranes: microbiology, Pseudomonas fluorescens: enzymology, Pseudomonas fluorescens: metabolism, Acetone, Aldehyde-Lyases, benzaldehyde lyase, carboligation, enzyme membrane reactor, enantioselective, C-C-coupling, enzyme kinetics |
| جغرافية الموضوع: | DE |
| الوصف: | Benzaldehyde lyase (BAL; E.C. 4.1.2.38) from Pseudomonas fluorescens Biovar I catalyzes the reversible formation of benzoins from aromatic aldehydes, and, moreover, the coupling of aromatic with aliphatic aldehydes yielding derivatives of (R)-2-hydroxy-1-phenyl- propan-1-one (R)-HPPs), which are important chiral building blocks. In this paper, we report on the development of a reactor system that allows the selective production of substituted (R)-HPP-derivatives. The reaction systems yielding (R)-1-(3-chloro-phenyl)-2-hydroxy- propan-1-one, (R)-2-hydroxy-3-methoxy-1-(4-methoxy-phenyl)-propan-1-one, and (R)-2-hydroxy-3,3-dimethoxy-1-phenyl-propan-1-one were investigated. A kinetic model optimized by batch experiments was developed, for the description of both batch and continuously operated reactors. This model was used to describe the HPP production in a continuously operated enzyme membrane reactor. The reactor type used combines the advantages of high conversion and excellent selectivity with high space-time yields and total turnover numbers of up to ttn=43,000. Products were obtained in high yield on a gram scale. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/pmid:17029295; info:eu-repo/semantics/altIdentifier/issn/0006-3592; info:eu-repo/semantics/altIdentifier/wos/WOS:000244830500002; https://juser.fz-juelich.de/record/52864; https://juser.fz-juelich.de/search?p=id:%22PreJuSER-52864%22 |
| الاتاحة: | https://juser.fz-juelich.de/record/52864 https://juser.fz-juelich.de/search?p=id:%22PreJuSER-52864%22 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.B7BCE9BC |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |