Academic Journal
Screening and identification of multiple constituents and their metabolites of Zhi‐zi‐chi decoction in rat urine and bile by ultra‐high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry
| العنوان: | Screening and identification of multiple constituents and their metabolites of Zhi‐zi‐chi decoction in rat urine and bile by ultra‐high‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry |
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| المؤلفون: | Feng, Wei, Dong, Qiuju, Liu, Minyan, Li, Si, Liu, Ting, Wang, Xinguo, Niu, Liying |
| المساهمون: | Hebei College Education Science Foundation of China, Hebei Provincial Natural Science Foundation of China |
| المصدر: | Biomedical Chromatography ; volume 31, issue 10 ; ISSN 0269-3879 1099-0801 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2017 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Zhi‐zi‐chi decoction (ZZCD) is a classical formula widely used in Chinese clinical application. In the present study, a novel and efficient strategy has been developed for screening and identification of multiple constituents and their metabolites of ZZCD using ultra‐high‐performance liquid chromatography combined with triple time‐of‐flight mass spectrometry. The novel approach of an online data acquisition method dependent on multiple mass defect filter and dynamic background subtraction is combined with multiple data processing techniques. First, a total of 109 potential bioactive compounds were detected in ZZCD. Based on the same instrumental conditions, 100 compounds were found in rat biofluids after oral administration of ZZCD, including 61 original compounds of ZZCD as well as 39 metabolites. Conjugations with sulfate, glucuronate and amino acids were found as the predominant metabolic reaction of ZZCD. As more xenobiotics were detected in urine than those in bile were, it demonstrated that multiple components of ZZCD have undergone comprehensive renal excretion. This study reported the urinary and biliary excretion in rats after oral administration of ZZCD for the first time. The present study expands our knowledge about the constituents and metabolism of ZZCD, which could be very useful for further pharmacological and clinical studies of ZZCD. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/bmc.3978 |
| الاتاحة: | http://dx.doi.org/10.1002/bmc.3978 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbmc.3978 https://onlinelibrary.wiley.com/doi/full/10.1002/bmc.3978 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.B7B4DBB3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/bmc.3978 |
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