Dissertation/ Thesis
Treinamento funcional altera o perfil metabólico, mitocondrial e redox de células mononucleares de mulheres com síndrome metabólica ; Functional training modifies the metabolic profile, mitochondrial and redox of the mononuclear cells in women with metabolic syndrome
| العنوان: | Treinamento funcional altera o perfil metabólico, mitocondrial e redox de células mononucleares de mulheres com síndrome metabólica ; Functional training modifies the metabolic profile, mitochondrial and redox of the mononuclear cells in women with metabolic syndrome |
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| المؤلفون: | Rosa, Pamela Carvalho da |
| المساهمون: | Barcelos, Rômulo Pillon, http://lattes.cnpq.br/8887253904142575, Souza, Diego de, XXXXXXXXXXXXXXX, Puntel, Gustavo Orione, XXXXXXXXXXXXXX |
| بيانات النشر: | Universidade Federal de Santa Maria Brasil Bioquímica UFSM Bioquímica Toxicológica Centro de Ciências Naturais e Exatas |
| سنة النشر: | 2019 |
| المجموعة: | Manancial - Repositório Digital da UFSM (Universidade Federal de Santa Maria) |
| مصطلحات موضوعية: | Exercício físico, Metabolismo energético, Respiração mitocondrial, Estado Redox e PBMCs, Physical exercise, Energy metabolism, Mitochondrial respiratory, Redox state, PBMCs, CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
| الوصف: | Metabolic syndrome (MS) is a combination of metabolic abnormalities, such as hypertension, hyperglycemia, abdominal obesityand dyslipidemia. These conditions occur simultaneously and increase the risk for type 2 diabetes and cardiovascular diseases development, and may be related to mitochondrial and antioxidant defense system dysfunction, once those conditions increase reactive oxygen species (ROS) generation, reduction of oxidative phosphorylation and, consequently, lower ATP synthesis. In this sense, the development of therapeutic strategies, including lifestyle interventions, such as diet and regular physical exercises directed to mitochondria metabolism, might be relevant and important for MS treatment and prevention. Moreover, it is well-known the beneficial effects of exercise at mitochondrial metabolism and redox states, but its impact on peripheral blood mononuclear cells (PBMCs) from patients with MS remains unclear. Therefore, this study intends to determine whether exercise training changes the energy metabolism, mitochondrial complexes capability and oxidative stress th MS, and associate them to the exercise parameters. To this, untrained middle-age women over 45 years old, and with three risk factors related to MS were recruited. Participants performed a functional training protocol during the 12-weeks (3d/wk). Cells (PBMCs) were obtained from subject's blood collection with MS before training (baseline) and 72 hours after the end of the training protocol (to avoid acute exercise effects - after training). After isolation, PBMCs were used to measure the following parameters: lactate dehydrogenase (LDH) and citrate synthase (CS) activity related for energy metabolism, and the mitochondrial complexes respiratory capability (I-IV). ROS production was performed for dichlorofluorescein oxidation and hydrogen peroxide (H2O2) production, as well as catalase (CAT) activity associated endogenous antioxidant system. The training protocol produced an increase in PBMCs LDH levels, while CS activity remained ... |
| نوع الوثيقة: | thesis |
| وصف الملف: | application/pdf |
| اللغة: | Portuguese |
| Relation: | http://repositorio.ufsm.br/handle/1/21139 |
| الاتاحة: | http://repositorio.ufsm.br/handle/1/21139 |
| Rights: | Attribution-NonCommercial-NoDerivatives 4.0 International ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.B764F744 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |