Academic Journal
A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines
| العنوان: | A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines |
|---|---|
| المؤلفون: | Hailes, Helen C, Wang, Yu, Li, Yiwen, Ni, Yeke, Bucar, Dejan-Kresimir, Dalby, Paul A, Ward, John M, Jeffries, Jack |
| المصدر: | Catalysis Science & Technology (2024) (In press). |
| بيانات النشر: | Royal Society of Chemistry (RSC) |
| سنة النشر: | 2024 |
| المجموعة: | University College London: UCL Discovery |
| الوصف: | Transaminase enzymes are well established biocatalysts that are used in chemical synthesis due to their beneficial sustainability profile, regio- and stereoselectivity and substrate specificity. Here, the use of a wild-type Chromobacterium violaceum transaminase (CvTAm) in enzyme cascades revealed the formation of a novel hydroxystyryl pyridine product. Subsequent studies established it was a transaminase mediated reaction where it was exhibiting apparent aldolase reactivity. This promiscuous enzyme reaction mechanism was then explored using other wild-type transaminases and via the formation of CvTAm mutants. Application of one pot multi-step enzyme cascades was subsequently developed to produce a range of hydroxystyryl pyridines. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://discovery.ucl.ac.uk/id/eprint/10189515/1/d3cy01370g.pdf; https://discovery.ucl.ac.uk/id/eprint/10189515/ |
| الاتاحة: | https://discovery.ucl.ac.uk/id/eprint/10189515/1/d3cy01370g.pdf https://discovery.ucl.ac.uk/id/eprint/10189515/ |
| Rights: | open |
| رقم الانضمام: | edsbas.B7436760 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |