التفاصيل البيبلوغرافية
| العنوان: |
Adenosine triphosphate induces amorphous aggregation of amyloid β by increasing Aβ dynamics |
| المؤلفون: |
Kuramochi, Masahiro, Nakamura, Momoka, Takahashi, Hiroto, Komoriya, Tomoe, Takita, Teisuke, Pham, Ngan Thi Kim, Yasukawa, Kiyoshi, Yoshimune, Kazuaki |
| المساهمون: |
倉持, 昌弘, 中村, 桃佳, 髙橋, 大翔, 小森谷, 友絵, 滝田, 禎亮, 保川, 清, 吉宗, 一晃 |
| بيانات النشر: |
Springer Nature |
| سنة النشر: |
2024 |
| المجموعة: |
Kyoto University Research Information Repository (KURENAI) / 京都大学学術情報リポジトリ |
| مصطلحات موضوعية: |
Alzheimer's disease, Biological physics |
| الوصف: |
アルツハイマー病に関係するアミロイドβ1分子の凝集動態を観察. 京都大学プレスリリース. 2024-04-23. ; Amyloid β (Aβ) aggregates into two distinct fibril and amorphous forms in the brains of patients with Alzheimer’s disease. Adenosine triphosphate (ATP) is a biological hydrotrope that causes Aβ to form amorphous aggregates and inhibit fibril formation at physiological concentrations. Based on diffracted X-ray blinking (DXB) analysis, the dynamics of Aβ significantly increased immediately after ATP was added compared to those in the absence and presence of ADP and AMP, and the effect diminished after 30 min as the aggregates formed. In the presence of ATP, the β-sheet content of Aβ gradually increased from the beginning, and in the absence of ATP, the content increased rapidly after 180 min incubation, as revealed by a time-dependent thioflavin T fluorescence assay. Images of an atomic force microscope revealed that ATP induces the formation of amorphous aggregates with an average diameter of less than 100 nm, preventing fibrillar formation during 4 days of incubation at 37℃. ATP may induce amorphous aggregation by increasing the dynamics of Aβ, and as a result, the other aggregation pathway is omitted. Our results also suggest that DXB analysis is a useful method to evaluate the inhibitory effect of fibrillar formation. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| تدمد: |
2045-2322 |
| Relation: |
https://www.kyoto-u.ac.jp/ja/research-news/2024-04-23-0; http://hdl.handle.net/2433/287952; Scientific Reports; 14; 8134 |
| الاتاحة: |
http://hdl.handle.net/2433/287952 |
| Rights: |
© The Author(s) 2024 ; This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.B707F22B |
| قاعدة البيانات: |
BASE |
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