Report
Critical contribution of mitochondria in the development of cardiomyopathy linked to desminmutation
| العنوان: | Critical contribution of mitochondria in the development of cardiomyopathy linked to desminmutation |
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| المؤلفون: | Hovhannisyan, Yeranuhi, Li, Zhenlin, Callon, Domitille, Suspène, Rodolphe, Batoumeni, Vivien, Canette, Alexis, Blanc, Jocelyne, Hocini, Hakim, Lefebvre, Cécile, El-Jahrani, Nora, L’honoré, Aurore, Kordeli, Ekaterini, Fornes, Paul, Concordet, Jean-Paul, Tachdjian, Gérard, Rodriguez, Anne-Marie, Vartanian, Jean-Pierre, Béhin, Anthony, Wahbi, Karim, Joanne, Pierre, Agbulut, Onnik |
| المساهمون: | Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A-IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | https://hal.science/hal-04287052 ; 2023. |
| بيانات النشر: | CCSD |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | Beyond the observed alterations in cellular structure and mitochondria, the cellular mechanisms linking genetic mutations to the development of heart failure in patients affected by desmin defects remain unclear due, in part, to the lack of relevant human cardiomyocyte models. We investigated the role of mitochondria using cardiomyocytes derived from human induced pluripotent stem cells carrying the heterozygous DES E439K desmin mutation, that were either isolated from a patient or generated by gene editing. To increase physiological relevance, cells were either cultured on an anisotropic surface to obtain elongated and aligned cardiomyocytes, or as spheroids to create a micro- tissue. When applicable, results were confirmed with heart biopsies from the family harboring DES E439K mutation. We show that mutant cardiomyocytes reproduce critical defects in mitochondrial architecture, respiratory capacity and metabolic activity as observed in patient’s heart tissue. To challenge the pathological mechanism, normal mitochondria were transferred inside the mutant cardiomyocytes. This treatment restored mitochondrial and contractile functions. This work demonstrates the crucial role of mitochondrial abnormalities in the pathophysiology of desmin-related cardiomyopathy, and opens-up new potential therapeutic perspectives. |
| نوع الوثيقة: | report |
| اللغة: | English |
| Relation: | BIORXIV: 2023.09.14.557734 |
| DOI: | 10.1101/2023.09.14.557734 |
| الاتاحة: | https://hal.science/hal-04287052 https://hal.science/hal-04287052v1/document https://hal.science/hal-04287052v1/file/2023.09.14.557734v2.full.pdf https://doi.org/10.1101/2023.09.14.557734 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.B6EA5A5D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2023.09.14.557734 |
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