Academic Journal
Inducible excision of selectable marker gene from transgenic plants by the Cre/lox site-specific recombination system
| العنوان: | Inducible excision of selectable marker gene from transgenic plants by the Cre/lox site-specific recombination system |
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| المؤلفون: | Wang, Y, Chen, BJ, Hu, YL, Li, JF, Lin, ZP |
| المساهمون: | Lin, ZP (reprint author), Peking Univ, Coll Life Sci, Dept Biotechnol, Beijing 100871, Peoples R China., Peking Univ, Coll Life Sci, Dept Biotechnol, Beijing 100871, Peoples R China., NE Agr Univ, Coll Hort, Harbin 150030, Peoples R China. |
| المصدر: | PubMed ; SCI |
| بيانات النشر: | 转基因研究 |
| سنة النشر: | 2005 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | autoexcision, Cre/lox recombination system, heat shock treatment, plant expression vector, selectable marker gene, transgenic tobacco, T-DNAS, AGROBACTERIUM-TUMEFACIENS, TRANSIENT EXPRESSION, CRE RECOMBINASE, TOBACCO, GENOME, COTRANSFORMATION, TRANSFORMATION, PROMOTER, TCUP |
| الوصف: | In a plant transformation process, it is necessary to use marker genes that allow the selection of regenerated transgenic plants. However, selectable marker genes are generally superfluous once an intact transgenic plant has been established. Furthermore, they may cause regulatory difficulties for approving transgenic crop release and commercialization. We constructed a binary expression vector with the Cre/lox system with a view to eliminating a marker gene from transgenic plants conveniently. In the vector, recombinase gene cre under the control of heat shock promoter and selectable marker gene nptII under the control of CaMV35S promoter were placed between two loxP sites in direct orientation, while the gene of interest was inserted outside of the loxP sites. By using this vector, both cre and nptII genes were eliminated from most of the regenerated plants of primary transformed tobacco through heat shock treatment, while the gene of interest was retained and stably inherited. This autoexcision strategy, mediated by the Cre/lox system and subjected to heat shock treatment to eliminate a selectable marker gene, is easy to adopt and provides a promising approach to generate marker-free transgenic plants. ; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000232799800008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; SCI(E) ; PubMed ; 72 ; ARTICLE ; 5 ; 605-614 ; 14 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 0962-8819 |
| Relation: | TRANSGENIC RESEARCH.2005,14,(5),605-614.; 675294; http://hdl.handle.net/20.500.11897/199054; WOS:000232799800008 |
| DOI: | 10.1007/s11248-005-0884-9 |
| الاتاحة: | https://hdl.handle.net/20.500.11897/199054 https://doi.org/10.1007/s11248-005-0884-9 |
| رقم الانضمام: | edsbas.B6BECCFF |
| قاعدة البيانات: | BASE |
| تدمد: | 09628819 |
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| DOI: | 10.1007/s11248-005-0884-9 |