Academic Journal
MicroRNA-769-5p contributes to the proliferation, migration and invasion of hepatocellular carcinoma cells by attenuating RYBP
| العنوان: | MicroRNA-769-5p contributes to the proliferation, migration and invasion of hepatocellular carcinoma cells by attenuating RYBP |
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| المؤلفون: | Yao Xian, Liang Wang, Bowen Yao, Wei Yang, Huanye Mo, Lei Zhang, Kangsheng Tu |
| المصدر: | Biomedicine & Pharmacotherapy, Vol 118, Iss , Pp - (2019) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2019 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Hepatocellular carcinoma, miR-769-5p, RYBP, Proliferation, Tumor metastasis, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Hepatocellular carcinoma (HCC) is the commonest primary liver cancer with highly aggressive features. MicroRNAs (miRNAs) are demonstrated to play important roles in the tumorigenesis and progression of HCC. miR-769-5p is a recently identified cancer-associated miRNA. But, the expression level of miR-769-5p and its function in HCC are unexplored. In this study, we found that miR-769-5p expression was obviously increased in HCC samples compared to adjacent noncancerous liver tissues. Additionally, we revealed that miR-769-5p was over-expressed in HCC cells as compared with LO2 cells. Notably, HCC tissues from patients with tumor size ≥5 cm, venous infiltration and advanced tumor stages showed higher levels of miR-769-5p compared to those from corresponding controls. Interestingly, our data indicated that HCC patients highly expressing miR-769-5p had significant shorter survivals. Next, functional experiments verified that miR-769-5p knockdown markedly suppressed HCC cell proliferation, migration and invasion. Conversely, ectopic expression of miR-769-5p promoted these biological behaviors of Hep3B cells. Furthermore, depletion of miR-769-5p repressed the growth and metastasis of HCCLM3 cells in vivo. Importantly, miR-769-5p inversely modulated RING1 and YY1 binding protein (RYBP) by directly binding to 3’ untranslated region (UTR) in HCC cells. The expression of RYBP mRNA was down-regulated in HCC tissues and negatively correlated with miR-769-5p level. RYBP overexpression remarkably inhibited the proliferation, migration and invasion of HCCLM3 cells. Accordingly, knockdown of RYBP partially abolished miR-769-5p silencing-induced tumor suppressive effects on HCCLM3 cells. In summary, our study revealed the up-regulated expression of miR-769-5p, which contributed to HCC progression possibly by targeting RYBP. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0753-3322 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0753332219328768; https://doaj.org/toc/0753-3322; https://doaj.org/article/52a79890934c4a6984d7510befa1b2ae |
| DOI: | 10.1016/j.biopha.2019.109343 |
| الاتاحة: | https://doi.org/10.1016/j.biopha.2019.109343 https://doaj.org/article/52a79890934c4a6984d7510befa1b2ae |
| رقم الانضمام: | edsbas.B6B3CEE1 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 07533322 |
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| DOI: | 10.1016/j.biopha.2019.109343 |