Academic Journal
Dose-dependent effect of triiodothyronine on the chondrogenic differentiation of mesenchymal stem cells from the bone marrow of female rats
| العنوان: | Dose-dependent effect of triiodothyronine on the chondrogenic differentiation of mesenchymal stem cells from the bone marrow of female rats |
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| المؤلفون: | Assis, Higor A, Elert, Nathalia C, Azevedo, André Luiz B P, Braga, Iolanda S, Serakides, Rogéria, de Melo Ocarino, Natália, de Goes, Alfredo M, de Paula Careta, Francisco, Paneto, Greiciane G, da Silva, Adriana M A, Boeloni, Jankerle N |
| المساهمون: | Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| المصدر: | Journal of Pharmacy and Pharmacology ; volume 70, issue 1, page 89-100 ; ISSN 2042-7158 0022-3573 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2017 |
| الوصف: | Objectives Verify the in-vitro effect of triiodothyronine (T3) on the chondrogenic differentiation of female rat bone marrow mesenchymal stem cells (BMMSCs) over several time periods and at several doses. Methods CD54 + /CD73 + /CD90 + BMMSCs from Wistar female rats were cultured in chondrogenic medium with or without T3 (0.01; 1; 100; 1000 nm). At seven, 14 and 21 days, the cell morphology, chondrogenic matrix formation and expression of Sox9 and collagen II were evaluated. Key findings The dose of 100 nm did not alter the parameters evaluated in any of the periods studied. However, the 0.01 nm T3 dose improved the chondrogenic potential by increasing the chondrogenic matrix formation and expression of Sox9 and collagen II in at least one of the evaluated periods; the 1 nm T3 dose also improved the chondrogenic potential by increasing the chondrogenic matrix formation and the expression of collagen II in at least one of the evaluated periods. The 1000 nm T3 dose improved the chondrogenic potential by increasing the chondrogenic matrix formation and Sox9 expression in at least one of the evaluated periods. Conclusions T3 has a dose-dependent effect on the differentiation of BMMSCs from female rats. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/jphp.12842 |
| الاتاحة: | http://dx.doi.org/10.1111/jphp.12842 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjphp.12842 http://academic.oup.com/jpp/article-pdf/70/1/89/36590456/jphp12842.pdf |
| Rights: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| رقم الانضمام: | edsbas.B67FDAB1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/jphp.12842 |
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