التفاصيل البيبلوغرافية
| العنوان: |
piCAR and diCAR induce proliferation and granulocytic differentiation. |
| المؤلفون: |
Kyoko Nakajima (14304927), Zhongchuzi Shen (10175225), Masashi Miura (280977), Hideto Nakabayashi (14304930), Masahiro Kawahara (2620522) |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
Cell Biology, Genetics, Molecular Biology, Biotechnology, Immunology, Developmental Biology, Cancer, Hematology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, curing blood disorders, various cell types, exclusively activate picar, dicar may become, chimeric antigen receptor, myeloid cell proliferation, sequentially controlling proliferation, cell fate control, sequential control, blood cells, cell proliferation, cell therapy, cell surface, construct proliferation, %22">xlink ">, two antigens, successfully replaced, sequential induction, recently applied, rationally designed |
| الوصف: |
(A) Cell proliferation assay to examine piCAR-mediated proliferation. Cells were cultured for 3 days in the medium with no factor (-), various concentrations of FL-BSA, or 1 ng/ml IL-3. Cell proliferation levels were estimated with a colorimetric assay by measuring absorbance at 450 nm. The data are represented as mean ± SD (n = 3, biological replicates). (B) Cell proliferation arrest induced by diCAR. Cells were inoculated at 2 x 10 5 cells/ml and cultured for 3 days in the medium with no factor (-), 1 ng/ml IL-3, 10 ng/ml G-CSF, or 0.1 μg/ml DNP-BSA. Cell proliferation levels were estimated with a colorimetric assay by measuring absorbance at 450 nm. The data are represented as mean ± SD (n = 3, biological replicates). (C) Cell differentiation assay. Cells precultured with 1 ng/ml IL-3 or 1 μg/ml FL-BSA were washed and cultured with 1 ng/ml IL-3, 1 μg/ml FL-BSA, 10 ng/ml G-CSF, or 0.1 μg/ml DNP-BSA. On day 5, the cells were stained with PE-labeled rat IgG2b κ isotype control or PE-labeled anti-mouse CD11b. Parental 32Dcl3 cells were used as controls. Histograms in each panel are represented for three biological replicates stained with isotype control-PE (orange, light blue, and red lines) and for those stained with CD11b-PE (flaxen, dark green, and light green lines). (D) Median fluorescence intensities (MFI) were calculated for the histograms presented in (C), and MFI ratios (CD11b / isotype control) were shown as mean ± SD (n = 3, biological replicates). Student’s t -test (for parental cells) and one-way ANOVA followed by post-hoc Bonferroni’s multiple-comparison test (for piCAR+diCAR transduced cells) were used for comparing the means of the groups. *** p < 0.001; ** p < 0.01. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/piCAR_and_diCAR_induce_proliferation_and_granulocytic_differentiation_/21783246 |
| DOI: |
10.1371/journal.pone.0279409.g005 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0279409.g005 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.B6384979 |
| قاعدة البيانات: |
BASE |