Academic Journal
Hydroxytyrosol prevents reduction in liver activity of Δ-5 and Δ-6 desaturases, oxidative stress, and depletion in long chain polyunsaturated fatty acid content in different tissues of high-fat diet fed mice
| العنوان: | Hydroxytyrosol prevents reduction in liver activity of Δ-5 and Δ-6 desaturases, oxidative stress, and depletion in long chain polyunsaturated fatty acid content in different tissues of high-fat diet fed mice |
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| المؤلفون: | Rodrigo Valenzuela, Francisca Echeverria, Macarena Ortiz, Miguel Ángel Rincón-Cervera, Alejandra Espinosa, María Catalina Hernandez-Rodas, Paola Illesca, Alfonso Valenzuela, Luis A. Videla |
| المصدر: | Lipids in Health and Disease, Vol 16, Iss 1, Pp 1-16 (2017) |
| بيانات النشر: | BMC |
| سنة النشر: | 2017 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Hydroxytyrosol, Oxidative stress, Antioxidant capacity, Δ5/Δ6 Desaturase activity, LCPUFA, Liver and extrahepatic tissues, Nutritional diseases. Deficiency diseases, RC620-627 |
| الوصف: | Background Eicosapentaenoic acid (EPA, C20:5n-3), docosahexaenoic acid (DHA, C22:6n-3) and arachidonic acid (AA, C20:4n-6) are long-chain polyunsaturated fatty acids (LCPUFAs) with relevant roles in the organism. EPA and DHA are synthesized from the precursor alpha-linolenic acid (ALA, C18:3n-3), whereas AA is produced from linoleic acid (LA, C18:2n-6) through the action of Δ5 and Δ6-desaturases. High-fat diet (HFD) decreases the activity of both desaturases and LCPUFA accretion in liver and other tissues. Hydroxytyrosol (HT), a natural antioxidant, has an important cytoprotective effects in different cells and tissues. Methods Male mice C57BL/6 J were fed a control diet (CD) (10% fat, 20% protein, 70% carbohydrates) or a HFD (60% fat, 20% protein, 20% carbohydrates) for 12 weeks. Animals were daily supplemented with saline (CD) or 5 mg HT (HFD), and blood and the studied tissues were analyzed after the HT intervention. Parameters studied included liver histology (optical microscopy), activity of hepatic desaturases 5 and 6 (gas-liquid chromatography of methyl esters derivatives) and antioxidant enzymes (catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase by spectrophotometry), oxidative stress indicators (glutathione, thiobarbituric acid reactants, and the antioxidant capacity of plasma), gene expression assays for sterol regulatory element-binding protein 1c (SREBP-1c) (qPCR and ELISA), and LCPUFA profiles in liver, erythrocyte, brain, heart, and testicle (gas-liquid chromatography). Results HFD led to insulin resistance and liver steatosis associated with SREBP-1c upregulation, with enhancement in plasma and liver oxidative stress status and diminution in the synthesis and storage of n-6 and n-3 LCPUFAs in the studied tissues, compared to animals given control diet. HT supplementation significantly reduced fat accumulation in liver and plasma as well as tissue metabolic alterations induced by HFD. Furthermore, a normalization of desaturase activities, oxidative ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1476-511X |
| Relation: | http://link.springer.com/article/10.1186/s12944-017-0450-5; https://doaj.org/toc/1476-511X; https://doaj.org/article/490a264c4efe40a39bf6c0ae6ca67268 |
| DOI: | 10.1186/s12944-017-0450-5 |
| الاتاحة: | https://doi.org/10.1186/s12944-017-0450-5 https://doaj.org/article/490a264c4efe40a39bf6c0ae6ca67268 |
| رقم الانضمام: | edsbas.B634AE06 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1476511X |
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| DOI: | 10.1186/s12944-017-0450-5 |