التفاصيل البيبلوغرافية
| العنوان: |
Prostate Cancer Biomarkers of Biochemical Recurrence in Low-risk prostate Cancer patients |
| المؤلفون: |
Talaibek Borbiev, Kaitlyn Bejar, Denise Young, Yingjie Song, Jiji Jiang, Jacob Kagan, Sudhir Srivastava, Javier Hernandez, Gregory Chesnut, Isabell A. Sesterhenn, Robin J. Leach, Gyorgy Petrovics, Indu Kohaar |
| المساهمون: |
Medicine (MED), SOM |
| المصدر: |
Conference ; USU Research Day ; Bethesda, MD ; RITM0037770Borbiev2023Poster.pdf |
| بيانات النشر: |
Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814
University Archives, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
Prostatic Neoplasms |
| الوصف: |
Introduction: Radical prostatectomy (RP) is widely used in clinical practice to treat men with localized prostate cancer. However, up to 20% � 40% of patients after RP may develop biochemical recurrence, which is a prognostic indicator of subsequent disease progression to a metastatic stage. Along with PSA, there is a need for additional and early detection biomarkers of biochemical recurrence in prostate cancer patients. We hypothesized that tissue specimens from early-stage, low risk prostate cancer may harbor predictive prognostic signatures for biochemical recurrence after RP. In our earlier study, we had comprehensively evaluated and optimized the NanoString platform for prostate cancer gene expression analysis in formalin fixed paraffin embedded (FFPE) whole mounted prostate specimens. The goal of the study is to identify molecular markers of poor disease outcome utilizing differential mRNA expression of candidate markers from patients with BCR (biochemical recurrence) and non-BCR in an independent cohort of samples from the University of Texas Health Science Center at San Antonio (UTHSCA). Methods: This is a retrospective racially diverse cohort study based on 78 tumor-normal paired (N=156) archived whole mounted FFPE prostate samples, comprising of 21.6% of AA and 78.8% of CA men with a minimum clinical follow-up of 5 years. All cases had a low-grade disease (Gleason pattern 3+3 or 3+4) with no signs of future disease progression. The cohort included 28 patients with BCR, and 50 patients with no BCR. We performed an optimized NanoString analysis of custom designed 203-prostate cancer probe set to evaluate the association of markers with BCR outcome. Normalized nCounter gene expression data was analyzed using the NanoString based nSolver (v 4.0) platform. ERG gene expression was validated by optimized immunohistochemistry assay using CPDR ERG-MAb (9FY) on FFPE whole mounted prostate samples. Fisher exact and Wilcoxon-Mann-Whitney tests were used for categorical and continuous variables respectively. ... |
| نوع الوثيقة: |
other/unknown material |
| وصف الملف: |
pdf |
| اللغة: |
unknown |
| Relation: |
http://cdm16005.contentdm.oclc.org/cdm/ref/collection/p16005coll8/id/575 |
| الاتاحة: |
http://cdm16005.contentdm.oclc.org/cdm/ref/collection/p16005coll8/id/575 |
| Rights: |
U.S. Government ; The views presented here are those of the author and are not to be construed as official or reflecting the views of the Uniformed Services University of the Health Sciences, the Department of Defense or the U.S. Government. |
| رقم الانضمام: |
edsbas.B5D9F92A |
| قاعدة البيانات: |
BASE |