Academic Journal
The Two-Component System ChtRS Contributes to Chlorhexidine Tolerance in Enterococcus faecium
| العنوان: | The Two-Component System ChtRS Contributes to Chlorhexidine Tolerance in Enterococcus faecium |
|---|---|
| المؤلفون: | Guzmán Prieto, Ana M., Wijngaarden, Jessica, Braat, Johanna C., Rogers, Malbert R. C., Majoor, Eline, Brouwer, Ellen C., Zhang, Xinglin, Bayjanov, Jumamurat R., Bonten, Marc J. M., Willems, Rob J. L., van Schaik, Willem |
| المساهمون: | European Commission, ZonMw |
| المصدر: | Antimicrobial Agents and Chemotherapy ; volume 61, issue 5 ; ISSN 0066-4804 1098-6596 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2017 |
| الوصف: | Enterococcus faecium is one of the primary causes of nosocomial infections. Disinfectants are commonly used to prevent infections with multidrug-resistant E. faecium in hospitals. Worryingly, E. faecium strains that exhibit tolerance to disinfectants have already been described. We aimed to identify and characterize E. faecium genes that contribute to tolerance to the disinfectant chlorhexidine (CHX). We used a transposon mutant library, constructed in a multidrug-resistant E. faecium bloodstream isolate, to perform a genome-wide screen to identify genetic determinants involved in tolerance to CHX. We identified a putative two-component system (2CS), composed of a putative sensor histidine kinase (ChtS) and a cognate DNA-binding response regulator (ChtR), which contributed to CHX tolerance in E. faecium . Targeted chtR and chtS deletion mutants exhibited compromised growth in the presence of CHX. Growth of the chtR and chtS mutants was also affected in the presence of the antibiotic bacitracin. The CHX- and bacitracin-tolerant phenotype of E. faecium E1162 was linked to a unique, nonsynonymous single nucleotide polymorphism in chtR . Transmission electron microscopy showed that upon challenge with CHX, the Δ chtR and Δ chtS mutants failed to divide properly and formed long chains. Normal growth and cell morphology were restored when the mutations were complemented in trans . Morphological abnormalities were also observed upon exposure of the Δ chtR and Δ chtS mutants to bacitracin. The tolerance to both chlorhexidine and bacitracin provided by ChtRS in E. faecium highlights the overlap between responses to disinfectants and antibiotics and the potential for the development of cross-tolerance for these classes of antimicrobials. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/aac.02122-16 |
| DOI: | 10.1128/AAC.02122-16 |
| الاتاحة: | http://dx.doi.org/10.1128/aac.02122-16 https://journals.asm.org/doi/pdf/10.1128/AAC.02122-16 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.B5CBEA73 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/aac.02122-16 |
|---|