Academic Journal
NASCarD (Nanopore Adaptive Sampling with Carrier DNA): A Rapid, PCR-Free Method for SARS-CoV-2 Whole-Genome Sequencing in Clinical Samples
| العنوان: | NASCarD (Nanopore Adaptive Sampling with Carrier DNA): A Rapid, PCR-Free Method for SARS-CoV-2 Whole-Genome Sequencing in Clinical Samples |
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| المؤلفون: | Miguel A. Terrazos Miani, Loïc Borcard, Sonja Gempeler, Christian Baumann, Pascal Bittel, Stephen L. Leib, Stefan Neuenschwander, Alban Ramette |
| المصدر: | Pathogens, Vol 13, Iss 1, p 61 (2024) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | beta coronaviruses, SARS-CoV-2, whole-genome sequencing, next-generation sequencing, genomics, nanopore sequencing, Medicine |
| الوصف: | Whole-genome sequencing (WGS) represents the main technology for SARS-CoV-2 lineage characterization in diagnostic laboratories worldwide. The rapid, near-full-length sequencing of the viral genome is commonly enabled by high-throughput sequencing of PCR amplicons derived from cDNA molecules. Here, we present a new approach called NASCarD (Nanopore Adaptive Sampling with Carrier DNA), which allows a low amount of nucleic acids to be sequenced while selectively enriching for sequences of interest, hence limiting the production of non-target sequences. Using COVID-19 positive samples available during the omicron wave, we demonstrate how the method may lead to >99% genome completeness of the SARS-CoV-2 genome sequences within 7 h of sequencing at a competitive cost. The new approach may have applications beyond SARS-CoV-2 sequencing for other DNA or RNA pathogens in clinical samples. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2076-0817 |
| Relation: | https://www.mdpi.com/2076-0817/13/1/61; https://doaj.org/toc/2076-0817; https://doaj.org/article/f679f8d420324c5db1a59bce8793aeb6 |
| DOI: | 10.3390/pathogens13010061 |
| الاتاحة: | https://doi.org/10.3390/pathogens13010061 https://doaj.org/article/f679f8d420324c5db1a59bce8793aeb6 |
| رقم الانضمام: | edsbas.B5B6A172 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20760817 |
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| DOI: | 10.3390/pathogens13010061 |