Academic Journal
Establishment of a 3D-dynamic osteoblasts-osteoclasts co-culture model to simulate the jawbone microenvironment in vitro
| العنوان: | Establishment of a 3D-dynamic osteoblasts-osteoclasts co-culture model to simulate the jawbone microenvironment in vitro |
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| المؤلفون: | PENOLAZZI, Maria Letizia, LOLLI, Andrea, Sardelli, Luca, ANGELOZZI, MARCO, LAMBERTINI, Elisabetta, TROMBELLI, Leonardo, CIARPELLA, Francesca, VECCHIATINI, Renata, PIVA, Maria Roberta |
| المساهمون: | Penolazzi, Maria Letizia, Lolli, Andrea, Sardelli, Luca, Angelozzi, Marco, Lambertini, Elisabetta, Trombelli, Leonardo, Ciarpella, Francesca, Vecchiatini, Renata, Piva, Maria Roberta |
| سنة النشر: | 2016 |
| المجموعة: | Università degli Studi di Ferrara: CINECA IRIS |
| مصطلحات موضوعية: | 3D cell co-culture, MRONJ, Osteoblast, Osteoclast, Medicine (all), Biochemistry, Genetics and Molecular Biology (all), Pharmacology, Toxicology and Pharmaceutics (all) |
| الوصف: | Aims We aimed to establish a 3D osteoblasts/osteoclasts co-culture system requiring limited amounts of human primary cells and useful as platform to 1. recapitulate an "oral bone microenvironment" in healthy or pathological condition, and 2. produce potential implantable cell constructs for regeneration of jawbone which can be negatively affected by bisphosphonates (BPs). Main methods Osteoblasts from normal bone chips (hOBs) or from jawbone of patients taking BPs (hnOBs) were co-cultured with monocytes (hMCs) either in static (3D-C) or dynamic (3D-DyC) condition using the RCCS-4TM bioreactor for 3 weeks. Cell aggregates were characterized for viability, histological features and specific osteoclastic and osteogenic markers. Key findings In all tested conditions hOBs supported the formation of mature osteoclasts (hOCs), without differentiating agents or exogenous scaffolds. 3D-DyC condition associated with a ground based condition (Xg) rather than modeled microgravity (μXg) produced aggregates with high level of osteogenic markers including Osteopontin (OPN), Osterix (OSX), Runx2 and appreciable bone mineral matrix. hnOBs co-cultured with hMCs in 3D-Dyc/Xg condition generated OPN and mineral matrix positive aggregates. Significance We optimized a 3D co-culture system with a limited amount of cells preserving viability and functionality of bone cellular components and generating bone-like aggregates also by using cells from jawbone necrotic tissue. The feasibility to obtain from poor-quality bone sites viable osteoblasts able to form aggregates when co-cultured with hMCs, allows to study the development of autologous implantable constructs to overcome jawbone deficiency in patients affected by MRONJ (Medication-Related Osteonecrosis of the Jaws). |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/27015789; info:eu-repo/semantics/altIdentifier/wos/WOS:000375728500011; volume:152; firstpage:82; lastpage:93; numberofpages:12; journal:LIFE SCIENCES; http://hdl.handle.net/11392/2353764; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84962694619; https://www.sciencedirect.com/science/article/pii/S0024320516301515 |
| DOI: | 10.1016/j.lfs.2016.03.035 |
| الاتاحة: | http://hdl.handle.net/11392/2353764 https://doi.org/10.1016/j.lfs.2016.03.035 https://www.sciencedirect.com/science/article/pii/S0024320516301515 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.B5B43205 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.lfs.2016.03.035 |
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