Academic Journal
Immunocompromisation of wheat host by L-BSO and 2,4-DPA induces susceptibility to the fungal pathogen Fusarium oxysporum
| العنوان: | Immunocompromisation of wheat host by L-BSO and 2,4-DPA induces susceptibility to the fungal pathogen Fusarium oxysporum |
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| المؤلفون: | Sahu, Abhaya Kumar, Kumari, Punam, Mittra, Bhabatosh |
| المصدر: | Stress Biology ; volume 4, issue 1 ; ISSN 2731-0450 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2024 |
| الوصف: | Susceptibility is defined as the disruption of host defence systems that promotes infection or limits pathogenicity. Glutathione (GSH) is a major component of defence signalling pathways that maintain redox status and is synthesised by γ-glutamyl cysteine synthetase (γ-ECS). On the other hand, lignin acts as a barrier in the primary cell wall of vascular bundles (VBs) synthesised by phenylalanine ammonia-lyase (PAL) in the intracellular system of plants. In this study, we used two inhibitors, such as L-Buthionine-sulfoximine (BSO), which irreversibly inhibits γ-ECS, and 2,4-dichlorophenoxyacetic acid (DPA), which reduces PAL activity and leads to the induction of oxidative stress in wheat ( Triticum aestivum ) seedlings after exposure to Fusarium oxysporum . Seedlings treated with 1 mM L-BSO and 2,4-DPA showed high levels of hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA), carbonyl (CO) content, and low activity of antioxidative enzymes [superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR)] as compared to wild-type (WT) seedlings under F. oxysporum infection. Further, the content of reduced glutathione (RGSH), ascorbate (ASC), and lignin was decreased in BSO and DPA treated seedlings as compared to WT seedlings during Fusarium infection. Moreover, treatment with BSO and DPA significantly inhibited the relative activity of γ-ECS and PAL ( P ≤ 0.001) in WT seedlings during Fusarium infection, which led to disintegrated VBs and, finally, cell death. Our results demonstrate that inhibition of γ-ECS and PAL by BSO and DPA, respectively, disrupts the defence mechanisms of wheat seedlings and induces susceptibility to F. oxysporum . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1007/s44154-023-00137-7 |
| DOI: | 10.1007/s44154-023-00137-7.pdf |
| DOI: | 10.1007/s44154-023-00137-7/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1007/s44154-023-00137-7 https://link.springer.com/content/pdf/10.1007/s44154-023-00137-7.pdf https://link.springer.com/article/10.1007/s44154-023-00137-7/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.B586BFE3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s44154-023-00137-7 |
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