Academic Journal
Effect of prebiotic fermentation products from primary human gut microbiota on an in vitro intestinal model
| العنوان: | Effect of prebiotic fermentation products from primary human gut microbiota on an in vitro intestinal model |
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| المؤلفون: | Parente, Inês, Xavier, Miguel, Roupar, Dalila, Amado, Isabel R., Berni, Paulo, Botelho, C. M., Teixeira, J. A., Pastrana, Lorenzo, Nobre, Clarisse, Gonçalves, Catarina |
| بيانات النشر: | Elsevier 1 |
| سنة النشر: | 2022 |
| المجموعة: | Universidade of Minho: RepositóriUM |
| مصطلحات موضوعية: | Intestinal models, Human gut microbiota, Prebiotics, Fermentation, Primary faecal samples, Intestinal in vitro models, Science & Technology |
| الوصف: | Short chain fatty acids (SCFA) are hypothesised to play a key role in the microbiota-gut-brain crosstalk. However, studies that evaluate the effect of microbiota-targeted interventions, such as prebiotics, probiotics or diet are still sparse, particularly in humans. In this work, the products of fermentation of prebiotic-enriched media by bacteria from primary human faecal samples were tested on differentiated Caco-2/HT29-MTX co-cultures. The prebiotics raftilose and fructo-oligosaccharides (FOS) were tested, and the experimental conditions (contact time and minimal dilution) were optimised to avoid cytotoxicity. The optimised conditions did not compromise the intestinal epithelium integrity. In addition, none of the fermentation products caused an inflammatory response. The products of fermentation of FOS-enriched media showed a moderate protective effect against the formation of reactive oxygen species. This work provides an important basis for the development of in vitro models using a simple approach to evaluate host-gut microbiota interactions. ; This work was supported by the ColOsH project (POCI-01-0145-FEDER-030071) funded by the FCT, the MICRODIGEST project (grant agreement no. 037716) co-funded by the FCT and ERDF through COMPETE2020 and by the European Union’s Horizon 2020 research and innovation programme through the project GASTRIC, under the Marie Sklodowska-Curie grant agreement no. 101003440. This work was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. Dalila Roupar acknowledges the FCT for the Ph.D. Grant SFRH/BD/139884/2018. ; info:eu-repo/semantics/publishedVersion |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1756-4646 |
| Relation: | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT; info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F139884%2F2018/PT; https://www.journals.elsevier.com/journal-of-functional-foods; Parente, Inês; Xavier, Miguel; Roupar, Dalila; Amado, Isabel R.; Berni, P.; Botelho, Cláudia M.; Teixeira, José A.; Pastrana, Lorenzo; Nobre, Clarisse; Gonçalves, Catarina, Effect of prebiotic fermentation products from primary human gut microbiota on an in vitro intestinal model. Journal of Functional Foods, 96(105200), 2022; https://hdl.handle.net/1822/79111; 105200 |
| DOI: | 10.1016/j.jff.2022.105200 |
| الاتاحة: | https://hdl.handle.net/1822/79111 https://doi.org/10.1016/j.jff.2022.105200 |
| Rights: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.B568FFF7 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 17564646 |
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| DOI: | 10.1016/j.jff.2022.105200 |