Academic Journal
Does singlet oxygen activate cell death in Arabidopsis cell suspension cultures? Analysis of the early transcriptional defense responses to high light stress
| العنوان: | Does singlet oxygen activate cell death in Arabidopsis cell suspension cultures? Analysis of the early transcriptional defense responses to high light stress |
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| المؤلفون: | Gutiérrez Merino, Jorge, González Pérez, Sergio, García-García, Francisco, Lorenzo, Óscar, Arellano, Juan B. |
| بيانات النشر: | Landes Bioscience |
| سنة النشر: | 2011 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Acclimation, Arabidopsis cell cultures, Cell death, Chloroplasts, Defense responses, Hormone stimulus, OPDA, oxylipins, Reactive oxygen species, Singlet oxygen, Transcriptional profiling, High light, Photosystem II |
| الوصف: | Can Arabidopsis cell suspension cultures (ACSC) provide a useful working model to investigate genetically-controlled defense responses with signaling cascades starting in chloroplasts? In order to provide a convincing answer, we analysed the early transcriptional profile of Arabidopsis cells at high light (HL). The results showed that ACSC respond to HL in a manner that resembles the singlet oxygen (1O2)-mediated defense responses described for the conditional fluorescent (flu) mutant of Arabidopsis thaliana. The flu mutant is characterized by the accumulation of free protochlorophyllide (Pchlide) in plastids when put into darkness and the subsequent production of 1O2 when the light is on. In ACSC, 1O2 is produced in chloroplasts at HL when excess excitation energy flows into photosystem II (PSII). Other reactive oxygen species are also produced in ACSC at HL, but to a lesser extent. When the HL stress ceases, ACSC recovers the initial rate of oxygen evolution and cell growth continues. We can conclude that chloroplasts of ACSC are both photosynthetically active and capable of initiating 1O2-mediated signaling cascades that activate a broad range of genetically-controlled defense responses. The upregulation of transcripts associated with the biosynthesis and signaling pathways of OPDA (12-oxophytodienoic acid) and ethylene (ET) suggests that the activated defense responses at HL are governed by these two hormones. In contrast to the flu mutant, the 1O2-mediated defense responses were independent of the upregulation of EDS1 (enhanced disease susceptibility) required for the accumulation of salicylic acid (SA) and genetically-controlled cell death. Interestingly, a high correlation in transcriptional expression was also observed between ACSC at HL, and the aba1 and max4 mutants of Arabidopsis, characterized by defects in the biosynthesis pathways of abscisic acid (ABA) and strigolactones, respectively. ; This work was supported by Junta de Castilla y León (grant number CSI002A10-2). ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 22112448 |
| Relation: | http://www.landesbioscience.com/journals/psb/article/18264/; http://hdl.handle.net/10261/43843 |
| DOI: | 10.4161/psb.6.12.18264 |
| الاتاحة: | http://hdl.handle.net/10261/43843 https://doi.org/10.4161/psb.6.12.18264 |
| Rights: | open |
| رقم الانضمام: | edsbas.B5619959 |
| قاعدة البيانات: | BASE |
| تدمد: | 22112448 |
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| DOI: | 10.4161/psb.6.12.18264 |