Report
Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes
| العنوان: | Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes |
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| المؤلفون: | Roffay, Chloé, García-Arcos, Juan Manuel, Chapuis, Pierrik, López-Andarias, Javier, Schneider, Falk, Colom, Adai, Tomba, Caterina, Meglio, Ilaria Di, Dunsing, Valentin, Matile, Stefan, Roux, Aurélien, Mercier, Vincent |
| المساهمون: | Department of Biochemistry, University of Geneva, CH-1211 Geneva, Department of Organic Chemistry, University of Geneva, CH-1211 Geneva, Translational Imaging Center, University of Southern California, Los Angeles, California 90089, Biofisika Institute (CSIC, UPV/EHU), 48940 Leioa, INL - Dispositifs pour la Santé et l’Environnement (INL - DSE), Institut des Nanotechnologies de Lyon (INL), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), Université de Genève = University of Geneva (UNIGE), Swiss National Centre for Competence in Research Programme Chemical Biology (NCCR-Chemical Biology), ALTF 849-2020, LT000404/2021-L, PID2019-111096GA-I00, MCIU/AEI/FEDER MINECOG19/P66, RYC2018-024686-I, and Basque Government T1270-19, HFSP LT0058/2022-L, 51NF40-185898), 51NF40-182895, 200020 204175, SNSFERCAdvanced Grant TIMEUP, TMAG-2_209190, Swiss National Fund for Research GrantsN°310030_200793 and N°CRSII5_189996 |
| المصدر: | https://hal.science/hal-04240839 ; 2023. |
| بيانات النشر: | CCSD |
| سنة النشر: | 2023 |
| المجموعة: | HAL Lyon 1 (University Claude Bernard Lyon 1) |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | Measuring forces within living cells remains a technical challenge. We developed hydrophobic mechanosensing fluorescent probes called Flippers, whose fluorescence lifetime depends on lipid packing and can report on membrane tension. Here, we describe technical optimization of the probe imaging, and diverse characterizations in various biological and in vitro systems. We provide a guideline to measure biophysical parameters of cellular membranes by FLIM microscopy with Flipper probes, providing evidences that flippers can report long range forces in cells, tissues and organs i . |
| نوع الوثيقة: | report |
| اللغة: | English |
| Relation: | BIORXIV: 2022.09.28.509885 |
| DOI: | 10.1101/2022.09.28.509885 |
| الاتاحة: | https://hal.science/hal-04240839 https://hal.science/hal-04240839v1/document https://hal.science/hal-04240839v1/file/2022.09.28.509885v3.full.pdf https://doi.org/10.1101/2022.09.28.509885 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.B558355D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2022.09.28.509885 |
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