Academic Journal
The in vitro kinetics of the interactions between PEG-ylated magnetic-fluid-loaded liposomes and macrophages
| العنوان: | The in vitro kinetics of the interactions between PEG-ylated magnetic-fluid-loaded liposomes and macrophages |
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| المؤلفون: | Martina, Marie-Sophie, Nicolas, Valérie, Wilhelm, Claire, Ménager, Christine, Barratt, Gillian, Lesieur, Sylviane |
| المساهمون: | Equipe Physico-chimie des systèmes polyphasés, Institut Galien Paris-Sud (IGPS), Université Paris-Sud - Paris 11 (UP11)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Matière et Systèmes Complexes (MSC), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Liquides Ioniques et Interfaces Chargées (LI2C), Université Pierre et Marie Curie - Paris 6 (UPMC)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), This work was supported by a grant of the Ministry of Research in France. |
| المصدر: | ISSN: 0142-9612 ; Biomaterials ; https://hal.science/hal-00212128 ; Biomaterials, 2007, 28 (28), pp.4143-4153. ⟨10.1016/j.biomaterials.2007.05.025⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | [CHIM.INOR]Chemical Sciences/Inorganic chemistry, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging |
| الوصف: | International audience ; Binding and uptake kinetics of magnetic-fluid-loaded liposomes (MFL) by endocytotic cells were investigated in vitro on the model cell-line J774. MFL consisted of unilamellar phosphatidylcholine vesicles (mean hydrodynamic diameter close to 200 nm) encapsulating 8-nm nanocrystals of maghemite (γ-Fe2O3) and sterically stabilized by introducing 5 mol% of distearylphosphatidylcholine poly(ethylene glycol)2000 (DSPE-PEG2000) in the vesicle bilayer. The association processes with living macrophages were followed at two levels. On one hand, the lipid vesicles were imaged by confocal fluorescence microscopy. For this purpose 1 mol% of rhodamine-marked phosphatidylethanolamine was added to the liposome composition. On the other hand, the iron oxide particles associated with cells were independently quantified by magnetophoresis. All the experiments were similarly performed with PEG-ylated or conventional MFL to point out the role of polymer coating. The results showed cell association with both types of liposomes resulting from binding followed by endocytosis. Steric stabilization by PEG chains reduced binding efficiency limiting the amount of MFL internalized by the macrophages. In contrast, PEG coating did not change the kinetics of endocytosis which exhibited the same first-order rate constant for both conventional and PEG-ylated liposomes. Moreover, lipids and iron oxide particle uptakes were perfectly correlated, indicating that MFL vesicle structure and encapsulation rate were preserved upon cell penetration. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-00212128; https://hal.science/hal-00212128 |
| DOI: | 10.1016/j.biomaterials.2007.05.025 |
| الاتاحة: | https://hal.science/hal-00212128 https://doi.org/10.1016/j.biomaterials.2007.05.025 |
| Rights: | http://creativecommons.org/licenses/by/ |
| رقم الانضمام: | edsbas.B515070E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.biomaterials.2007.05.025 |
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