التفاصيل البيبلوغرافية
| العنوان: |
SDS-PAGE analysis of BGL secretion over time in clones with and without IRA1 N200D . |
| المؤلفون: |
Benjamin Offei (5537873), Stephanie Braun-Galleani (4028279), Anjan Venkatesh (11184090), William T. Casey (14265489), Kevin E. O’Connor (5697374), Kevin P. Byrne (14186221), Kenneth H. Wolfe (8728713) |
| سنة النشر: |
2022 |
| مصطلحات موضوعية: |
Microbiology, Cell Biology, Genetics, Molecular Biology, Evolutionary Biology, Developmental Biology, Infectious Diseases, Virology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Mathematical Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, week batch cultures, weaker cell wall, reduced cell viability, pichia pastoris )<, div >< p, amino acid substitution, second qtl mapped, major qtl mapped, single genetic background, pichia pastoris <, higher protein secretion, secreted heterologous proteins, komagataella phaffii <, isolates doubled β, secreted proteins, komagataella phaffii, heterologous protein, phaffii < |
| الوصف: |
Proteins were extracted from supernatants of 100-ml shake-flask cultures at 96 hours, 120 hours, 144 hours, and 168 hours. Comparisons are between CBS_PGAP (3 technical replicates), CBS_BGL9 (3 technical replicates), and ICs (4 independently clones in which the IRA1 N200D variant was introduced by genome editing). The arrow indicates BGL, which migrates at its expected size of 120 kDa. L, molecular size standards. Due to a problem during gel loading, the sample from the fourth IC at 144 hours was split between 2 lanes. The cultures used for this experiment were the same ones used in Fig 6 . (PDF) |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/SDS-PAGE_analysis_of_BGL_secretion_over_time_in_clones_with_and_without_i_IRA1_i_sup_N200D_sup_/21733443 |
| DOI: |
10.1371/journal.pbio.3001877.s007 |
| الاتاحة: |
https://doi.org/10.1371/journal.pbio.3001877.s007 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.B50FF055 |
| قاعدة البيانات: |
BASE |